Difference between revisions of "Part:BBa K4195065"

Revision as of 15:23, 10 October 2022

ori_i

Biology

This sequence is the detection target taken from the literature(1).

Ribozyme ENabled Detection of RNA (RENDR)
RENDR is a high-performing, plug-and-play RNA-sensing platform(1). RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments (Fig. 1). Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When bound with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.

Fig. 1 Schematic illustration of RENDR.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4195065 short</partinfo>
-none
+===Biology===
+This sequence is the detection target taken from the literature(<i>1</i>).
+<b>Ribozyme ENabled Detection of RNA (RENDR)</b>
+<br>
+RENDR is a high-performing, plug-and-play RNA-sensing platform(<i>1</i>). RENDR utilizes a split variant of the <i>Tetrahymena thermophila</i> ribozyme by synthetically splitting it into two non-functional fragments (Fig. 1). Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When bound with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.
+<br>
+[[File:T--XMU-China--RENDR.png|400px]]
+<br>
+<b>Fig. 1 Schematic illustration of RENDR.</b>
 <!-- Add more about the biology of this part here