Difference between revisions of "Help:Plasmid Backbone Availability 2009"
 
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<span style="color:red; font-weight:bold; font-size:115%;"> **EDITING IN PROGRESS** </span>
 
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The following is a list of plasmid backbones that we have included as part of the 2009 distribution kit, most of which have P1010 and J04450. However, if you are having trouble with these, we have also included alternate sources for the plasmids.
 
The following is a list of plasmid backbones that we have included as part of the 2009 distribution kit, most of which have P1010 and J04450. However, if you are having trouble with these, we have also included alternate sources for the plasmids.
  
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!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="100"|'''P1010'''
 
!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="100"|'''P1010'''
 
!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="175"|'''Alt. Location'''
 
!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="175"|'''Alt. Location'''
−
!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="125"|'''Insert'''
+
!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="125"|'''Insert''' <span style="font-size:75%;">(click for QC)</span>
  
 
|- valign='top'
 
|- valign='top'
−
|'''BBa_I51020''' || YES || YES || NO ||
+
|'''pSB1A3''' || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1A3 Kit Plate 1, well 1K]</span> || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1A3 Kit Plate 1, well 3K]</span> || Kit Plate 2, well 10G [https://parts.igem.org/partsdb/get_part.cgi?id=4586&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I732205 BBa_I732205]
 +
|-
 +
|'''pSB1AC3''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1AC3 Kit Plate 1, well 11A]</span> || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1AC3 Kit Plate 1, well 11C]</span> || Kit Plate 2, well 22M [https://parts.igem.org/partsdb/get_part.cgi?id=5485&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_M1001 BBa_M1001]
 
|-
 
|-
−
|'''BBa_I716101''' || YES [?] || YES [?] || NO ||
+
|'''pSB1AK3''' || NO || NO || Kit Plate 3, well 20A [https://parts.igem.org/partsdb/get_part.cgi?id=4942&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K143053 BBa_K143053]
 
|-
 
|-
−
|'''BBa_J63009''' || YES || YES [?] || Kit Plate 3, well 2M [https://parts.igem.org/partsdb/get_part.cgi?id=7521&show=1 [more]]|| [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K165018 BBa_K165018]
+
|'''pSB1AT3''' || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1AT3 Kit Plate 1, well 15A]</span> || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1AT3 Kit Plate 1, well 15C]</span> || Kit Plate 3, well 1B [https://parts.igem.org/partsdb/get_part.cgi?id=5483&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K115001 BBa_K115001]
−
|-
+
−
|'''BBa_J63010''' || NO || NO || Kit Plate 3, well 2K [https://parts.igem.org/partsdb/get_part.cgi?id=7585&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K165001 BBa_K165001]
+
 
|-
 
|-
 
|'''pSB1A10''' || NO || NO || NO ||
 
|'''pSB1A10''' || NO || NO || NO ||
 
|-
 
|-
−
|'''pSB1A3''' || YES || YES || Kit Plate 2, well 10G [https://parts.igem.org/partsdb/get_part.cgi?id=4586&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I732205 BBa_I732205]
+
|'''pSB4A5''' || NO || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB4A5 Kit Plate 1, well 3M]</span> || NO ||
−
|-
+
−
|'''pSB4A5''' || NO || YES || NO ||
+
 
|-  
 
|-  
 
|'''pSB6A1''' || NO || NO || Kit Plate 2, well 14G [https://parts.igem.org/partsdb/get_part.cgi?id=10245&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I739001 BBa_I739001]
 
|'''pSB6A1''' || NO || NO || Kit Plate 2, well 14G [https://parts.igem.org/partsdb/get_part.cgi?id=10245&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I739001 BBa_I739001]
 
|-
 
|-
−
|'''pSB1AC3''' || YES [?] || YES [?] || Kit Plate 2, well 22M [https://parts.igem.org/partsdb/get_part.cgi?id=5485&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_M1001 BBa_M1001]
+
|'''pSB1C3''' || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1C3 Kit Plate 1, well 5A]</span> || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1C3 Kit Plate 1, well 5E]</span> || NO ||
−
|-
+
−
|'''pSB1AK3''' || NO || YES [?] || Kit Plate 3, well 20A [https://parts.igem.org/partsdb/get_part.cgi?id=4942&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K143053 BBa_K143053]
+
−
|-
+
−
|'''pSB1AK8''' || YES [?] || NO || Kit Plate 2, well 13A [https://parts.igem.org/partsdb/get_part.cgi?id=7524&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I712006 BBa_I712006]
+
−
|-
+
−
|'''pSB1AT3''' || YES || YES [?] || Kit Plate 3, well 1B [https://parts.igem.org/partsdb/get_part.cgi?id=5483&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K115001 BBa_K115001]
+
−
|-
+
−
|'''pSB1C3''' || YES || YES || NO ||
+
 
|-  
 
|-  
−
|'''pSB3C5''' || NO || YES || NO ||
+
|'''pSB3C5''' || NO || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB3C5 Kit Plate 1, well 5G]</span> || NO ||
 
|-  
 
|-  
−
|'''pSB4C5''' || NO || YES || Kit Plate 3, well 5F [https://parts.igem.org/partsdb/get_part.cgi?id=8151&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K098010 BBa_K098010]
+
|'''pSB4C5''' || NO || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB4C5 Kit Plate 1, well 5I]</span> || Kit Plate 3, well 5F [https://parts.igem.org/partsdb/get_part.cgi?id=8151&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K098010 BBa_K098010]
 
|-  
 
|-  
−
|'''pSB1K3''' || YES [?] || NO || NO ||
+
|'''pSB1K3''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1K3 Kit Plate 1, well 7A]</span> || NO || NO ||
 
|-
 
|-
 
|'''pSB2K3''' || NO || NO || Kit Plate 2, well 11B [https://parts.igem.org/partsdb/get_part.cgi?id=4584&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_E0033 BBa_E0033]
 
|'''pSB2K3''' || NO || NO || Kit Plate 2, well 11B [https://parts.igem.org/partsdb/get_part.cgi?id=4584&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_E0033 BBa_E0033]
Line 54: Line 42:
 
|'''pSB3K3''' || NO || NO || Kit Plate 2, well 15L [https://parts.igem.org/partsdb/get_part.cgi?id=4739&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I714891 BBa_I714891]
 
|'''pSB3K3''' || NO || NO || Kit Plate 2, well 15L [https://parts.igem.org/partsdb/get_part.cgi?id=4739&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I714891 BBa_I714891]
 
|-
 
|-
−
|'''pSB4K5''' || YES [?] || YES [?] || Kit Plate 2, well 17H [https://parts.igem.org/partsdb/get_part.cgi?id=8325&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_T9003 BBa_T9003]
+
|'''pSB4K5''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB4K5 Kit Plate 1, well 7G]</span> || NO || Kit Plate 2, well 17H [https://parts.igem.org/partsdb/get_part.cgi?id=8325&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_T9003 BBa_T9003]
 
|-
 
|-
−
|'''pSB1T3''' || YES [?] || NO || NO ||
+
|'''pSB1AK8''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1AK8 Kit Plate 1, well 13C]</span> || NO || Kit Plate 2, well 13A [https://parts.igem.org/partsdb/get_part.cgi?id=7524&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I712006 BBa_I712006]
 
|-
 
|-
−
|'''pSB3T5''' || YES [?] || YES [?] || Kit Plate 2, well 19A [https://parts.igem.org/partsdb/get_part.cgi?id=8615&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I715045 BBa_I715045]
+
|'''pSB1T3''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1T3 Kit Plate 1, well 9A]</span> || NO || NO ||
 +
|-
 +
|'''pSB3T5''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB3T5 Kit Plate 1, well 9C]</span> || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB3T5 Kit Plate 1, well 9G]</span> || Kit Plate 2, well 19A [https://parts.igem.org/partsdb/get_part.cgi?id=8615&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I715045 BBa_I715045]
 
|-
 
|-
 
|'''pSB4T5''' || NO || NO || NO ||
 
|'''pSB4T5''' || NO || NO || NO ||
 +
|-
 +
|'''BBa_I51020''' || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I51020 Kit Plate 1, well 1A]</span> || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I51020 Kit Plate 1, well 3A]</span> || NO ||
 +
|-
 +
|'''BBa_I716101''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I716101 Kit Plate 1, well 1C]</span> || NO || NO ||
 +
|-
 +
|'''BBa_J63009''' || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=BBa_J63009 Kit Plate 1, well 1E]</span> || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=BBa_J63009 Kit Plate 1, well 3E]</span> || Kit Plate 3, well 2M [https://parts.igem.org/partsdb/get_part.cgi?id=7521&show=1 [more]]|| [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K165018 BBa_K165018]
 +
|-
 +
|'''BBa_J63010''' || NO || NO || Kit Plate 3, well 2K [https://parts.igem.org/partsdb/get_part.cgi?id=7585&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K165001 BBa_K165001]
 
|-
 
|-
 
|}
 
|}
  
  
−
*If you decide to use an alternate location for the plasmid, keep in mind that after transforming and miniprepping you should use PCR to [http://openwetware.org/wiki/Making_construction_plasmid make the construction plasmid]. This step will produce PCR product containing the plasmid backbone with no insert and no uncut, circular plasmid, and can then be cut with the restriction enzymes for use.
+
*If you decide to use an alternate location for the plasmid, keep in mind that after [[Help:Transformation_Protocol|transforming]] and miniprepping you should use PCR to [[Help:Construction_Plasmid|make the construction plasmid]]. This step will produce PCR product containing the plasmid backbone with no insert and no uncut, circular plasmid, and can then be cut with the restriction enzymes for use.
  
  
 
We will continue to update this page with more information and details, including more comprehensive quality control information and alternate locations.
 
We will continue to update this page with more information and details, including more comprehensive quality control information and alternate locations.
 +
 +
 +
=Current Plasmid Backbone Status=
 +
''Updated on 10/5/09''
 +
{|border="2" cellpadding="1" align="center" style="text-align:center; border-style:solid; border-color:black; border-collapse:collapse;"
 +
 +
!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="50"|'''Well'''
 +
!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="100"|'''Insert'''
 +
!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="100"|'''Backbone'''
 +
!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="75"|'''Resistance'''
 +
!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="125"|'''Current Status'''
 +
!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="100"|'''Gel Results'''
 +
!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="100"|'''Seq Insert'''
 +
!style="background:#C0C0C0; color: #00602D; border-bottom:2px solid #000;" width="100"|'''Seq Plasmid'''
 +
|- valign='top'
 +
|-
 +
| 1A ||  BBa_J04450 || ''' BBa_I51020''' || A || '''Original 2009''' || WRONG ||  ||  ||
 +
|-
 +
| 1B ||  BBa_J04450 || ''' BBa_I716101''' || A || '''REASSEMBLED''' ||  ||  ||  ||
 +
|-
 +
| 1C ||  BBa_J04450 || ''' BBa_J63009''' || A || '''Original 2009''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 1D ||  BBa_J04450 || ''' BBa_J63010''' || A || '''Original 2009''' || WRONG ||  ||  ||
 +
|-
 +
| 1E ||  BBa_J04450 || ''' pSB1A10''' || A || '''REASSEMBLED''' || CONFIRMED ||  ||  ||
 +
|-
 +
| 1F ||  BBa_J04450 || ''' pSB1A3''' || A || '''REASSEMBLED''' || CONFIRMED ||  ||  ||
 +
|-
 +
| 1G ||  BBa_J04450 || ''' pSB4A5''' || A || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 1H ||  BBa_J04450 || ''' pSB6A1''' || A || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 2A ||  BBa_P1010 || ''' BBa_I51020''' || A || '''Original 2009''' || WRONG ||  ||  ||
 +
|-
 +
| 2B ||  BBa_P1010 || ''' BBa_I716101''' || A || '''REASSEMBLED''' ||  ||  ||  ||
 +
|-
 +
| 2C ||  BBa_P1010 || ''' BBa_J63009''' || A || '''REASSEMBLED''' ||  || CONFIRMED ||  ||
 +
|-
 +
| 2D ||  BBa_P1010 || ''' BBa_J63010''' || A || '''REASSEMBLED''' ||  ||  ||  ||
 +
|-
 +
| 2E ||  BBa_P1010 || ''' pSB1A10''' || A || '''REASSEMBLED''' || CONFIRMED ||  ||  ||
 +
|-
 +
| 2F ||  BBa_P1010 || ''' pSB1A3''' || A || '''Original 2009''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 2G ||  BBa_I52001 || ''' pSB4A5''' || A || '''Original 2009''' || CONFIRMED ||  ||  ||
 +
|-
 +
| 2H ||  BBa_P1010 || ''' pSB6A1''' || A || '''REASSEMBLED''' || CONFIRMED ||  ||  ||
 +
|-
 +
| 3A ||  BBa_J04450 || ''' pSB1C3''' || C || '''Original 2009''' || CONFIRMED ||  ||  ||
 +
|-
 +
| 3B ||  BBa_J04450 || ''' pSB3C5''' || C || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 3C ||  BBa_P1010 || ''' pSB1C3''' || C || '''Original 2009''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 3D ||  BBa_I52001 || ''' pSB3C5''' || C || '''Original 2009''' || CONFIRMED ||  ||  ||
 +
|-
 +
| 3E ||  BBa_I52002 || ''' pSB4C5''' || C || '''Original 2009''' || CONFIRMED ||  ||  ||
 +
|-
 +
| NA ||  BBa_J04450 || ''' pSB4C5''' || C || '''REASSEMBLED''' || CONFIRMED ||  ||  ||
 +
|-
 +
| 4A ||  BBa_J04450 || ''' pSB1K3''' || K || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 4B ||  BBa_J04450 || ''' pSB2K3''' || K || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 4C ||  BBa_J04450 || ''' pSB3K3''' || K || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 4D ||  BBa_J04450 || ''' pSB4K5''' || K || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 4E ||  BBa_P1010 || ''' pSB1K3''' || K || '''REASSEMBLED''' ||  || CONFIRMED ||  ||
 +
|-
 +
| 4F ||  BBa_P1010 || ''' pSB2K3''' || K || '''REASSEMBLED''' ||  ||  ||  ||
 +
|-
 +
| 4G ||  BBa_P1010 || ''' pSB3K3''' || K || '''REASSEMBLED''' ||  ||  ||  ||
 +
|-
 +
| 4H ||  BBa_P1010 || ''' pSB4K5''' || K || '''REASSEMBLED''' ||  || CONFIRMED ||  ||
 +
|-
 +
| 5A ||  BBa_J04450 || ''' pSB1T3''' || T || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 5B ||  BBa_J04450 || ''' pSB3T5''' || T || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 5C ||  BBa_P1010 || ''' pSB1T3''' || T || '''REASSEMBLED''' ||  ||  ||  ||
 +
|-
 +
| 5D ||  BBa_I52001 || ''' pSB3T5''' || T || '''REASSEMBLED''' ||  || CONFIRMED ||  ||
 +
|-
 +
| 5E ||  BBa_I52001 || ''' pSB4T5''' || T || '''in progress''' ||  ||  ||  ||
 +
|-
 +
| NA ||  BBa_J04450 || ''' pSB4T5''' || T || '''in progress''' ||  ||  ||  ||
 +
|-
 +
| 6A ||  BBa_J04450 || ''' pSB1AC3''' || AC || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 6B ||  BBa_P1010 || ''' pSB1AC3''' || AC || '''REASSEMBLED''' || CONFIRMED ||  ||  ||
 +
|-
 +
| 7A ||  BBa_J04450 || ''' pSB1AK3''' || AK || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 7B ||  BBa_J04450 || ''' pSB1AK8''' || AK || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 7C ||  BBa_P1010 || ''' pSB1AK3''' || AK || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 7D ||  BBa_P1010 || ''' pSB1AK8''' || AK || '''REASSEMBLED''' ||  ||  ||  ||
 +
|-
 +
| 8A ||  BBa_J04450 || ''' pSB1AT3''' || AT || '''Original 2009''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
| 8B ||  BBa_P1010 || ''' pSB1AT3''' || AT || '''REASSEMBLED''' || CONFIRMED || CONFIRMED ||  ||
 +
|-
 +
|}

Latest revision as of 18:03, 5 October 2009

The following is a list of plasmid backbones that we have included as part of the 2009 distribution kit, most of which have P1010 and J04450. However, if you are having trouble with these, we have also included alternate sources for the plasmids.


  • YES - The plasmid is available and correct
  • YES[?] - The plasmid is available and correct, with the exception of one inconsistency in the QC results which should not be an issue
  • NO - The plasmid is either unavailable or we have found that it carries an error


Plasmid J04450 P1010 Alt. Location Insert (click for QC)
pSB1A3 YES
Kit Plate 1, well 1K 		YES
Kit Plate 1, well 3K 		Kit Plate 2, well 10G [more] BBa_I732205
pSB1AC3 YES [?]
Kit Plate 1, well 11A 		YES [?]
Kit Plate 1, well 11C 		Kit Plate 2, well 22M [more] BBa_M1001
pSB1AK3 NO NO Kit Plate 3, well 20A [more] BBa_K143053
pSB1AT3 YES
Kit Plate 1, well 15A 		YES [?]
Kit Plate 1, well 15C 		Kit Plate 3, well 1B [more] BBa_K115001
pSB1A10 NO NO NO
pSB4A5 NO YES
Kit Plate 1, well 3M 		NO
pSB6A1 NO NO Kit Plate 2, well 14G [more] BBa_I739001
pSB1C3 YES
Kit Plate 1, well 5A 		YES
Kit Plate 1, well 5E 		NO
pSB3C5 NO YES
Kit Plate 1, well 5G 		NO
pSB4C5 NO YES
Kit Plate 1, well 5I 		Kit Plate 3, well 5F [more] BBa_K098010
pSB1K3 YES [?]
Kit Plate 1, well 7A 		NO NO
pSB2K3 NO NO Kit Plate 2, well 11B [more] BBa_E0033
pSB3K3 NO NO Kit Plate 2, well 15L [more] BBa_I714891
pSB4K5 YES [?]
Kit Plate 1, well 7G 		NO Kit Plate 2, well 17H [more] BBa_T9003
pSB1AK8 YES [?]
Kit Plate 1, well 13C 		NO Kit Plate 2, well 13A [more] BBa_I712006
pSB1T3 YES [?]
Kit Plate 1, well 9A 		NO NO
pSB3T5 YES [?]
Kit Plate 1, well 9C 		YES [?]
Kit Plate 1, well 9G 		Kit Plate 2, well 19A [more] BBa_I715045
pSB4T5 NO NO NO
BBa_I51020 YES
Kit Plate 1, well 1A 		YES
Kit Plate 1, well 3A 		NO
BBa_I716101 YES [?]
Kit Plate 1, well 1C 		NO NO
BBa_J63009 YES
Kit Plate 1, well 1E 		YES [?]
Kit Plate 1, well 3E 		Kit Plate 3, well 2M [more] BBa_K165018
BBa_J63010 NO NO Kit Plate 3, well 2K [more] BBa_K165001


  • If you decide to use an alternate location for the plasmid, keep in mind that after transforming and miniprepping you should use PCR to make the construction plasmid. This step will produce PCR product containing the plasmid backbone with no insert and no uncut, circular plasmid, and can then be cut with the restriction enzymes for use.


We will continue to update this page with more information and details, including more comprehensive quality control information and alternate locations.


Current Plasmid Backbone Status

Updated on 10/5/09

Well Insert Backbone Resistance Current Status Gel Results Seq Insert Seq Plasmid
1A BBa_J04450 BBa_I51020 A Original 2009 WRONG
1B BBa_J04450 BBa_I716101 A REASSEMBLED
1C BBa_J04450 BBa_J63009 A Original 2009 CONFIRMED CONFIRMED
1D BBa_J04450 BBa_J63010 A Original 2009 WRONG
1E BBa_J04450 pSB1A10 A REASSEMBLED CONFIRMED
1F BBa_J04450 pSB1A3 A REASSEMBLED CONFIRMED
1G BBa_J04450 pSB4A5 A REASSEMBLED CONFIRMED CONFIRMED
1H BBa_J04450 pSB6A1 A REASSEMBLED CONFIRMED CONFIRMED
2A BBa_P1010 BBa_I51020 A Original 2009 WRONG
2B BBa_P1010 BBa_I716101 A REASSEMBLED
2C BBa_P1010 BBa_J63009 A REASSEMBLED CONFIRMED
2D BBa_P1010 BBa_J63010 A REASSEMBLED
2E BBa_P1010 pSB1A10 A REASSEMBLED CONFIRMED
2F BBa_P1010 pSB1A3 A Original 2009 CONFIRMED CONFIRMED
2G BBa_I52001 pSB4A5 A Original 2009 CONFIRMED
2H BBa_P1010 pSB6A1 A REASSEMBLED CONFIRMED
3A BBa_J04450 pSB1C3 C Original 2009 CONFIRMED
3B BBa_J04450 pSB3C5 C REASSEMBLED CONFIRMED CONFIRMED
3C BBa_P1010 pSB1C3 C Original 2009 CONFIRMED CONFIRMED
3D BBa_I52001 pSB3C5 C Original 2009 CONFIRMED
3E BBa_I52002 pSB4C5 C Original 2009 CONFIRMED
NA BBa_J04450 pSB4C5 C REASSEMBLED CONFIRMED
4A BBa_J04450 pSB1K3 K REASSEMBLED CONFIRMED CONFIRMED
4B BBa_J04450 pSB2K3 K REASSEMBLED CONFIRMED CONFIRMED
4C BBa_J04450 pSB3K3 K REASSEMBLED CONFIRMED CONFIRMED
4D BBa_J04450 pSB4K5 K REASSEMBLED CONFIRMED CONFIRMED
4E BBa_P1010 pSB1K3 K REASSEMBLED CONFIRMED
4F BBa_P1010 pSB2K3 K REASSEMBLED
4G BBa_P1010 pSB3K3 K REASSEMBLED
4H BBa_P1010 pSB4K5 K REASSEMBLED CONFIRMED
5A BBa_J04450 pSB1T3 T REASSEMBLED CONFIRMED CONFIRMED
5B BBa_J04450 pSB3T5 T REASSEMBLED CONFIRMED CONFIRMED
5C BBa_P1010 pSB1T3 T REASSEMBLED
5D BBa_I52001 pSB3T5 T REASSEMBLED CONFIRMED
5E BBa_I52001 pSB4T5 T in progress
NA BBa_J04450 pSB4T5 T in progress
6A BBa_J04450 pSB1AC3 AC REASSEMBLED CONFIRMED CONFIRMED
6B BBa_P1010 pSB1AC3 AC REASSEMBLED CONFIRMED
7A BBa_J04450 pSB1AK3 AK REASSEMBLED CONFIRMED CONFIRMED
7B BBa_J04450 pSB1AK8 AK REASSEMBLED CONFIRMED CONFIRMED
7C BBa_P1010 pSB1AK3 AK REASSEMBLED CONFIRMED CONFIRMED
7D BBa_P1010 pSB1AK8 AK REASSEMBLED
8A BBa_J04450 pSB1AT3 AT Original 2009 CONFIRMED CONFIRMED
8B BBa_P1010 pSB1AT3 AT REASSEMBLED CONFIRMED CONFIRMED