Difference between revisions of "Part:BBa K4195179"
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===Biology=== | ===Biology=== | ||
| − | This sequence is a conserved region of toxin gene ''pirA''.[1] It’s used as the detection target of RENDR system.<br/> | + | This sequence is a conserved region of toxin gene ''pirA''.<sup>[1]</sup> It’s used as the detection target of RENDR system.<br/> |
'''Ribozyme ENabled Detection of RNA (RENDR)'''<br/> | '''Ribozyme ENabled Detection of RNA (RENDR)'''<br/> | ||
RENDR is a high-performing, plug-and-play RNA-sensing platform. RENDR utilizes a split variant of the ''Tetrahymena thermophila'' ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.<br/> | RENDR is a high-performing, plug-and-play RNA-sensing platform. RENDR utilizes a split variant of the ''Tetrahymena thermophila'' ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.<br/> | ||
Revision as of 15:09, 10 October 2022
Biology
This sequence is a conserved region of toxin gene pirA.[1] It’s used as the detection target of RENDR system.
Ribozyme ENabled Detection of RNA (RENDR)
RENDR is a high-performing, plug-and-play RNA-sensing platform. RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.
Fig. 1 Schematic illustration of RENDR.
Usage and Design
This part is used as the target of the RENDR detection system. For toxin pirA, we designed BBa_K4195141, BBa_K4195142, BBa_K4195145, BBa_K4195146, BBa_K4195156, BBa_K4195157, BBa_K4195160, BBa_K4195161, BBa_K4195168, BBa_K4195169, BBa_K4195172, BBa_K4195173. Other related parts are as followings: BBa_K4195151, BBa_K4195183, BBa_K4195184, BBa_K4195185, BBa_K4195186.
Reference
[1] Lazarte, J., Kim, Y. R., Lee, J. S., Chun, J. H., Kim, S. W., Jung, J. W., Kim, J., Kayansamruaj, P., Thompson, K. D., Kim, H., & Jung, T. S. (2021). Passive Immunization with Recombinant Antibody VLRB-PirAvp/PirBvp-Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp. Vaccines, 9(1), 55. https://doi.org/10.3390/vaccines9010055