Difference between revisions of "Part:BBa K4165011"
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<partinfo>BBa_K4165011 short</partinfo>
 
<partinfo>BBa_K4165011 short</partinfo>
  
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Ubiquitin-conjugating E2 ligase has a role in the ubiquitination cascade for protein degradation.
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Ubiquitin-conjugating E2 ligase that has a role in the ubiquitination cascade for protein degradation.
  
 
===Usage and Biology===
 
===Usage and Biology===
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encodes a member of the E2 ubiquitin-conjugating enzyme family and is involved in the ubiquitin-proteasome pathway, this ligase will be used in the project in the Trim21 system as a type of ligase essential for the ubiquitin cascade
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This part encodes for the UBE2N enzyme which is a member of the E2 ubiquitin-conjugating enzyme family, it acts as the second enzyme in the ubiquitination cascade to receive the thioesterified ubiquitin from the E1 active site. Finally, The E3 ubiquitin ligase then promotes the transfer of ubiquitin onto the substrate by binding to both the protein substrate and the E2-bound ubiquitin. It interacts with the UBE2V2 (E2 ligase) to catalyze the formation of polyubiquitin chains. This protein may also be involved in DNA post replication repair, according to studies that have been done on mice.
  
 
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===Refrences===
 
===Refrences===
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1- Komander D. (2009). The emerging complexity of protein ubiquitination. Biochemical Society transactions, 37(Pt 5), 937–953. https://doi.org/10.1042/BST0370937
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1. UBE2N ubiquitin conjugating enzyme E2 N [Homo sapiens (human)] - Gene - NCBI. (2022).
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2. Pharos: Ubiquitin-conjugating enzyme E2 N (Tchem). (2022).  
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2- David, Y., Ziv, T., Admon, A., & Navon, A. (2010). The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines. The Journal of biological chemistry, 285(12), 8595–8604. https://doi.org/10.1074/jbc.M109.089003
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3. Kleiger, G., & Mayor, T. (2014). Perilous journey: a tour of the ubiquitin-proteasome system. Trends in cell biology, 24(6), 352-359.
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3- Li, J., Xu, C., Zhang, J., Jin, C., Shi, X., Zhang, C., ... & Xu, L. (2021). Identification of miRNA-target gene pairs in the parietal and frontal lobes of the brain in patients with Alzheimer’s Disease using bioinformatic analyses. Neurochemical Research, 46(4), 964-979.
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Revision as of 14:43, 10 October 2022


UBE2N

Ubiquitin-conjugating E2 ligase that has a role in the ubiquitination cascade for protein degradation.

Usage and Biology

This part encodes for the UBE2N enzyme which is a member of the E2 ubiquitin-conjugating enzyme family, it acts as the second enzyme in the ubiquitination cascade to receive the thioesterified ubiquitin from the E1 active site. Finally, The E3 ubiquitin ligase then promotes the transfer of ubiquitin onto the substrate by binding to both the protein substrate and the E2-bound ubiquitin. It interacts with the UBE2V2 (E2 ligase) to catalyze the formation of polyubiquitin chains. This protein may also be involved in DNA post replication repair, according to studies that have been done on mice.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 221
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Refrences

1. UBE2N ubiquitin conjugating enzyme E2 N [Homo sapiens (human)] - Gene - NCBI. (2022). 2. Pharos: Ubiquitin-conjugating enzyme E2 N (Tchem). (2022). 3. Kleiger, G., & Mayor, T. (2014). Perilous journey: a tour of the ubiquitin-proteasome system. Trends in cell biology, 24(6), 352-359.