Difference between revisions of "Part:BBa K4245160"
 
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<partinfo>BBa_K4245160 short</partinfo>
 
<partinfo>BBa_K4245160 short</partinfo>
 
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This part is the Padlock spacer sequence, designed to stabilize the Broccoli fluorescent RNA aptamer ([https://parts.igem.org/Part:BBa_K3380153 BBa_K3380153]) in the RCT padlock probes, hsa-miR-1-3p RCT Padlock Probe ([https://parts.igem.org/Part:BBa_K4245121 BBa_K4245121]), hsa-mir-133a-3p RCT Padlock Probe ([https://parts.igem.org/Part:BBa_K4245201 BBa_K4245201]), and hsa-miR-208a-3p RCT Padlock Probe ([https://parts.igem.org/Part:BBa_K4245208 BBa_K4245208]).  
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This part is the Padlock spacer sequence, designed to stabilize the Broccoli fluorescent RNA aptamer (<partinfo>BBa_K3380153</partinfo>) in the RCT padlock probes, hsa-miR-1-3p RCT Padlock Probe (<partinfo>BBa_K4245121</partinfo>), hsa-mir-133a-3p RCT Padlock Probe (<partinfo>BBa_K4245205</partinfo>), and hsa-miR-208a-3p RCT Padlock Probe (<partinfo>BBa_K4245208</partinfo>).  
 
===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 14:38, 10 October 2022


Padlock spacer sequence
This part is the Padlock spacer sequence, designed to stabilize the Broccoli fluorescent RNA aptamer (BBa_K3380153) in the RCT padlock probes, hsa-miR-1-3p RCT Padlock Probe (BBa_K4245121), hsa-mir-133a-3p RCT Padlock Probe (BBa_K4245205), and hsa-miR-208a-3p RCT Padlock Probe (BBa_K4245208).

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]