Difference between revisions of "Part:BBa K4245210"
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This part is the T7 regulated miRNA1-broccoli-spacer, designed to test the efficacy of our part, <partinfo>BBa_K4245201</partinfo>, the hsa-miR-1-3p RCT Padlock Probe. We were unsure if the inclusion of a spacer sequence (<partinfo>BBa_K4245160</partinfo>) affected the folding of the broccoli aptamer (<partinfo>BBa_K3380153</partinfo>). The part was designed to imitate the transcript produced when Rolling Circle Transcription (RCT) is applied to hsa-miR-1-3p RCT Padlock Probe. | This part is the T7 regulated miRNA1-broccoli-spacer, designed to test the efficacy of our part, <partinfo>BBa_K4245201</partinfo>, the hsa-miR-1-3p RCT Padlock Probe. We were unsure if the inclusion of a spacer sequence (<partinfo>BBa_K4245160</partinfo>) affected the folding of the broccoli aptamer (<partinfo>BBa_K3380153</partinfo>). The part was designed to imitate the transcript produced when Rolling Circle Transcription (RCT) is applied to hsa-miR-1-3p RCT Padlock Probe. | ||
Revision as of 14:35, 10 October 2022
T7 regulated miRNA1-broccoli-spacer
This part is the T7 regulated miRNA1-broccoli-spacer, designed to test the efficacy of our part, BBa_K4245201, the hsa-miR-1-3p RCT Padlock Probe. We were unsure if the inclusion of a spacer sequence (BBa_K4245160) affected the folding of the broccoli aptamer (BBa_K3380153). The part was designed to imitate the transcript produced when Rolling Circle Transcription (RCT) is applied to hsa-miR-1-3p RCT Padlock Probe.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 137
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]