Difference between revisions of "Part:BBa K4461013:Experience"
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===Applications of BBa_K4461013=== | ===Applications of BBa_K4461013=== | ||
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| + | If the plasmid contained glpABC-mCherry with tag, there will be an EcoRI site that we used to insert tag by enzyme digestion and ligation, or it is a plasmid contained mut-glpABC-mCherry, which doesn't have an EcoRI site on it. Therefore, we digested them with EcoRI and BamHI, and the one being digested into 2 bands is correct. | ||
| + | [[File:Glp-mCherry-tag-check.jpg|400px|thumb|center|Fig1. After EcoRI/BamHI enzyme digestion, we found the correct plasmid (glpABC-mCherry-tag1). ]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 14:32, 10 October 2022
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how you used this part and how it worked out.
Applications of BBa_K4461013
If the plasmid contained glpABC-mCherry with tag, there will be an EcoRI site that we used to insert tag by enzyme digestion and ligation, or it is a plasmid contained mut-glpABC-mCherry, which doesn't have an EcoRI site on it. Therefore, we digested them with EcoRI and BamHI, and the one being digested into 2 bands is correct.
User Reviews
UNIQe1b87fb7f211a0c4-partinfo-00000000-QINU UNIQe1b87fb7f211a0c4-partinfo-00000001-QINU