Difference between revisions of "Part:BBa K4156078"
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It is mainly regulated by the transcriptional activator (FNR). In the absence of oxygen, the FNR binds to the [4Fe-4S]2+ cluster to generate a transcriptionally active homodimer. However, in the presence of oxygen, the [4Fe-4S]2+ cluster is degraded and the FNR dimer dissociates into inactive monomers.
 
It is mainly regulated by the transcriptional activator (FNR). In the absence of oxygen, the FNR binds to the [4Fe-4S]2+ cluster to generate a transcriptionally active homodimer. However, in the presence of oxygen, the [4Fe-4S]2+ cluster is degraded and the FNR dimer dissociates into inactive monomers.
  
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===Characterization===
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==hypoxia induced promoter testing==
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We constructed a hypoxia reporter consisting of the hypoxia-inducible promoter pPepT+mRFP. To test its performance, we added reporter in different chassis organisms.Fig 1,2 indicates that pPepT induces the expression of the downstream gene mRFP with the decrease of O2. Thus, it can be seen that the hypoxia reporter can work properly.
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                <img style="max-width:700px;" src="https://static.igem.wiki/teams/4156/wiki/part/2-1.png" alt="control">
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                <figcaption><b>Figure 1:</b> Induction of downstream gene mRFP expression under hypoxic and normoxic conditions in different chassis organisms over 48h.</figcaption>
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<figure style="text-align:center;">
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                <img style="max-width:700px;" src="https://static.igem.wiki/teams/4156/wiki/part/2-2-5-2.png" alt="control">
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                <figcaption><b>Figure 2:</b> Induction of downstream gene mRFP expression over time by a hypoxia reporter consisting of pPepT+mRFP under hypoxic and normoxic conditions.</figcaption>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 14:00, 10 October 2022


-- No description --

pPepT is a hypoxia-sensing promoter, designed to sense oxygen.

Usage and Biology

It is mainly regulated by the transcriptional activator (FNR). In the absence of oxygen, the FNR binds to the [4Fe-4S]2+ cluster to generate a transcriptionally active homodimer. However, in the presence of oxygen, the [4Fe-4S]2+ cluster is degraded and the FNR dimer dissociates into inactive monomers.

Characterization

hypoxia induced promoter testing

We constructed a hypoxia reporter consisting of the hypoxia-inducible promoter pPepT+mRFP. To test its performance, we added reporter in different chassis organisms.Fig 1,2 indicates that pPepT induces the expression of the downstream gene mRFP with the decrease of O2. Thus, it can be seen that the hypoxia reporter can work properly.

control
Figure 1: Induction of downstream gene mRFP expression under hypoxic and normoxic conditions in different chassis organisms over 48h.

control
Figure 2: Induction of downstream gene mRFP expression over time by a hypoxia reporter consisting of pPepT+mRFP under hypoxic and normoxic conditions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]