Difference between revisions of "Part:BBa K4245201:Experience"
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| + | Rolling Circle Transcription (RCT) was run with this part but proved to be unsuccessful. The products of RCT are long RNA strands composed of repeating complementary sequences of the used padlock probe. Therefore, one way in which the success of RCT can be determined is by running the RCT products on an agarose gel, since a fluorescent band very close to the wells would indicate the presence of extremely long nucleic acid strands. However, the products of RCT did not appear on a 1% agarose gel (see Fig. 1). | ||
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===Applications of BBa_K4245201=== | ===Applications of BBa_K4245201=== | ||
Revision as of 13:42, 10 October 2022
Rolling Circle Transcription (RCT) was run with this part but proved to be unsuccessful. The products of RCT are long RNA strands composed of repeating complementary sequences of the used padlock probe. Therefore, one way in which the success of RCT can be determined is by running the RCT products on an agarose gel, since a fluorescent band very close to the wells would indicate the presence of extremely long nucleic acid strands. However, the products of RCT did not appear on a 1% agarose gel (see Fig. 1).
Applications of BBa_K4245201
User Reviews
UNIQ7a6dfb1447b03a10-partinfo-00000000-QINU UNIQ7a6dfb1447b03a10-partinfo-00000001-QINU