Difference between revisions of "Part:BBa K4165110"

Revision as of 13:35, 10 October 2022

HTRA Binding Peptide 16

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

decameric peptide, Binds specifically to PDZ domain of HTRA1 Protein with ΔG of 2.2 ± 0.2 kcal/mol.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations = 0, clashscore = 56.25, molprobity = 4.33, ramachandran_favored = 12.5, ramachandran_outliers = 75, Qmean_4 = -6.45183, Qmean6 = -5.513969

Modeling

                 Figure 1.: 3D Model of H1P peptide Modelled by AlphaFold2.0 displayed in Pyomol

References

Rey, J., Breiden, M., Lux, V., Bluemke, A., Steindel, M., Ripkens, K., ... & Ehrmann, M. (2022). An allosteric HTRA1-calpain 2 complex with restricted activation profile. Proceedings of the National Academy of Sciences, 119(14), e2113520119.

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-decameric peptide, Binds specifically to PDZ domain of HTRA1 Protein with G of 2.2 ± 0.2 kcal/mol.
+decameric peptide, Binds specifically to PDZ domain of HTRA1 Protein with ΔG of 2.2 ± 0.2 kcal/mol.
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