Difference between revisions of "Part:BBa K228009"
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This part contains AraC protein generator(<partinfo>BBa_K228008</partinfo>) and Pbad promoter(<partinfo>BBa_I13453</partinfo>). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose. | This part contains AraC protein generator(<partinfo>BBa_K228008</partinfo>) and Pbad promoter(<partinfo>BBa_I13453</partinfo>). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose. | ||
| − | This promoter | + | This promoter can be placed upstream of a GFP gene to character its induction curve. We cultured the bacteria until the value of OD600 reached 0.4 and then added different concentration of arabinose. The process of inducement lasted two hours. We used flowcytometry to test the fluorescence in order to acquire the induction curve, which is shown in the below graph: |
[[Image:PKU_AraC.jpg|600px]] | [[Image:PKU_AraC.jpg|600px]] | ||
Revision as of 01:49, 3 October 2009
AraC protein(reversed sequence) and Pbad promoter
This part contains AraC protein generator(BBa_K228008) and Pbad promoter(BBa_I13453). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose.
This promoter can be placed upstream of a GFP gene to character its induction curve. We cultured the bacteria until the value of OD600 reached 0.4 and then added different concentration of arabinose. The process of inducement lasted two hours. We used flowcytometry to test the fluorescence in order to acquire the induction curve, which is shown in the below graph:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1274
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1214
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]