Difference between revisions of "Part:BBa K4461004"

 
 
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<partinfo>BBa_K4461004 short</partinfo>
 
<partinfo>BBa_K4461004 short</partinfo>
  
The ansB promoter encodes for the L-asparaginase II, which is involved in the asp metabolism.  
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The ansB encodes for the L-asparaginase II, which is involved in the asp metabolism.  
 
After complete glucose depletion at the late log phase, bacteria catabolize amino acids for energy and carbon.
 
After complete glucose depletion at the late log phase, bacteria catabolize amino acids for energy and carbon.
 
The ansB promoter is down-regulated by the fis, which is mainly expressed in the lag phase and early log phase, also it is co-activated by FNR and CRP, which represent the anaerobic growth and the depletion of glucose, respectively.  
 
The ansB promoter is down-regulated by the fis, which is mainly expressed in the lag phase and early log phase, also it is co-activated by FNR and CRP, which represent the anaerobic growth and the depletion of glucose, respectively.  
  
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[[File:AnsB mRNA expression.png|400px|thumb|center|This is the ansB mRNA expression-Time graph.]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:05, 10 October 2022


ansB promoter

The ansB encodes for the L-asparaginase II, which is involved in the asp metabolism. After complete glucose depletion at the late log phase, bacteria catabolize amino acids for energy and carbon. The ansB promoter is down-regulated by the fis, which is mainly expressed in the lag phase and early log phase, also it is co-activated by FNR and CRP, which represent the anaerobic growth and the depletion of glucose, respectively.

This is the ansB mRNA expression-Time graph.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]