Difference between revisions of "Part:BBa K4218016"
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| + | <html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><meta http-equiv="Content-Style-Type" content="text/css" /><meta name="generator" content="Aspose.Words for .NET 17.1.0.0" /><title></title></head><body><div><p style="margin-top:0pt; margin-bottom:22.5pt; text-align:justify; line-height:18pt; background-color:#ffffff"><span style="font-family:Arial; font-size:16pt; font-weight:bold">Double </span><span style="font-family:Arial; font-size:16pt; font-weight:bold">t</span><span style="font-family:Arial; font-size:16pt; font-weight:bold">oehold Switch</span></p><p style="margin-top:0pt; margin-bottom:22.5pt; text-align:justify; line-height:18pt; background-color:#ffffff"><img src="https://static.igem.org/mediawiki/parts/3/39/YiYe_China_4.png" width="554" height="285" alt="" style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /></p><p style="margin-top:0pt; margin-bottom:22.5pt; text-align:justify; line-height:18pt; background-color:#ffffff"><span style="font-family:Arial; font-size:10pt">The Toehold switch represses translation through its hairpin structure, which is </span><span style="font-family:Arial; font-size:10pt">hairpinned</span><span style="font-family:Arial; font-size:10pt"> behind the RBS and the initiation codon (AUG). The trigger sequence is an RNA sequence that is fully antisense complementary to the trigger strand of the Toehold switch and is essential for initiating translation. When these two linear RNAs are complementary, the stem loop will open and the ribosome will bind to the RBS, recognize the start codon and begin </span><span style="font-family:Arial; font-size:10pt">translation.In</span><span style="font-family:Arial; font-size:10pt"> the following, we combined the toehold of part BBa_K3577001 and part BBa_K3822000 to form double toehold.</span></p><p style="margin-top:0pt; margin-bottom:0pt; text-align:justify; widows:0; orphans:0; font-size:10.5pt"><span style="font-family:'等线'"> </span></p></div></body></html> | ||
Latest revision as of 13:03, 10 October 2022
Double toehold Switch
The Toehold switch represses translation through its hairpin structure, which is hairpinned behind the RBS and the initiation codon (AUG). The trigger sequence is an RNA sequence that is fully antisense complementary to the trigger strand of the Toehold switch and is essential for initiating translation. When these two linear RNAs are complementary, the stem loop will open and the ribosome will bind to the RBS, recognize the start codon and begin translation.In the following, we combined the toehold of part BBa_K3577001 and part BBa_K3822000 to form double toehold.