Difference between revisions of "Part:BBa K4461001"

Latest revision as of 13:01, 10 October 2022

cyoA promoter

cyoA code for the cytochrome bo3 oxidase which is involved in the aerobic respiratory chain. Fis activates cyoA expression during lag and early log phase. The expression of cyoA is increased under acidic growth conditions in aerobiosis. In conclusion, this promoter is activated during lag and early log phase due to the fis regulation, and the expression is decrease after that for both arcA and FNR regulation under anaerobic conditions. The rise after 4h is caused by the GadE regulation. However, we thought that, compared with the late log expression promoter “ansB”, it won’t be a big trouble.

This is a cyoA mRNA expression-time gragh.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4461001 short</partinfo>
-cyoA code for the cytochrome bo3 oxidase is involved in the aerobic respiratory chain.
+cyoA code for the cytochrome bo3 oxidase which is involved in the aerobic respiratory chain.
-Fis promoter activates cyoA promoter expression during lag and early log phase
+Fis activates cyoA expression during lag and early log phase. The expression of cyoA is increased under acidic growth conditions in aerobiosis.
-The expression of cyoA is increased under acidic growth conditions in aerobiosis.
+In conclusion, this promoter is activated during lag and early log phase due to the fis regulation, and the expression is decrease after that for both arcA and FNR regulation under anaerobic conditions. The rise after 4h is caused by the GadE regulation. However, we thought that, compared with the late log expression promoter “ansB”, it won’t be a big trouble.
+[[File:cyoa-rna-expression.png|200px|thumb|left|This is a cyoA mRNA expression-time gragh.]]