Difference between revisions of "Part:BBa K4245160"
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<partinfo>BBa_K4245160 short</partinfo> | <partinfo>BBa_K4245160 short</partinfo> | ||
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− | This part is the Padlock spacer sequence, designed to stabilize the Broccoli fluorescent RNA aptamer ([https://parts.igem.org/Part:BBa_K3380153 BBa_K3380153]) in the RCT padlock probes, hsa-miR-1-3p RCT Padlock Probe (BBa_K4245121), hsa-mir-133a-3p RCT Padlock Probe ( | + | This part is the Padlock spacer sequence, designed to stabilize the Broccoli fluorescent RNA aptamer ([https://parts.igem.org/Part:BBa_K3380153 BBa_K3380153]) in the RCT padlock probes, hsa-miR-1-3p RCT Padlock Probe ([https://parts.igem.org/Part:BBa_K4245121 BBa_K4245121]), hsa-mir-133a-3p RCT Padlock Probe (([https://parts.igem.org/Part:BBa_K4245201 BBa_K4245201])), and hsa-miR-208a-3p RCT Padlock Probe (([https://parts.igem.org/Part:BBa_K4245208 BBa_K4245208])). |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 12:53, 10 October 2022
Padlock spacer sequence
This part is the Padlock spacer sequence, designed to stabilize the Broccoli fluorescent RNA aptamer (BBa_K3380153) in the RCT padlock probes, hsa-miR-1-3p RCT Padlock Probe (BBa_K4245121), hsa-mir-133a-3p RCT Padlock Probe ((BBa_K4245201)), and hsa-miR-208a-3p RCT Padlock Probe ((BBa_K4245208)).
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]