Difference between revisions of "Part:BBa K4461002:Experience"

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===Applications of BBa_K4461002===
 
===Applications of BBa_K4461002===
  
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[[File:gDNA total.jpg|200px|thumb|center|Fig.1 We cloned a larger fragment that included nirBDC promoter from E.coli K-12 MG1655 gDNA. It is about 446 bp.]]
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[[File:gDNA total.jpg|400px|thumb|center|Fig.1 We cloned a larger fragment that included nirBDC promoter from E.coli K-12 MG1655 gDNA. It is about 446 bp.]]
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[[File:2nd total.jpg|400px|thumb|center|Fig.2  After adding suffix and prefix to the promoter, the nirBDC promoter part will be 357bp.]]
  
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[[File:2nd total.jpg|200px|thumb|center|Fig.2  After adding suffix and prefix to the promoter, the nirBDC promoter part will be 357bp.]]
 
  
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We construct a device BBa_K4461501, with backbone pSB1C3. Then, we test the FP intensity and OD for it and Fig.3 is the result.
 
We construct a device BBa_K4461501, with backbone pSB1C3. Then, we test the FP intensity and OD for it and Fig.3 is the result.
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[[File: NirBDC-mCerulean.png |200px|thumb|center|Fig.3  This graph shows that the mCerulean expressed since the sixth hour.]]
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[[File: NirBDC-mCerulean.png |400px|thumb|center|Fig.3  This graph shows that the mCerulean expressed since the sixth hour.]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 12:33, 10 October 2022


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4461002

Fig.1 We cloned a larger fragment that included nirBDC promoter from E.coli K-12 MG1655 gDNA. It is about 446 bp.
Fig.2 After adding suffix and prefix to the promoter, the nirBDC promoter part will be 357bp.


We construct a device BBa_K4461501, with backbone pSB1C3. Then, we test the FP intensity and OD for it and Fig.3 is the result.


Fig.3 This graph shows that the mCerulean expressed since the sixth hour.

User Reviews

UNIQbfc9e5d49c129df9-partinfo-00000000-QINU UNIQbfc9e5d49c129df9-partinfo-00000001-QINU