Difference between revisions of "Part:BBa K4461002:Experience"

Latest revision as of 12:33, 10 October 2022

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4461002

Fig.1 We cloned a larger fragment that included nirBDC promoter from E.coli K-12 MG1655 gDNA. It is about 446 bp.

Fig.2 After adding suffix and prefix to the promoter, the nirBDC promoter part will be 357bp.

We construct a device BBa_K4461501, with backbone pSB1C3. Then, we test the FP intensity and OD for it and Fig.3 is the result.

Fig.3 This graph shows that the mCerulean expressed since the sixth hour.

User Reviews

UNIQ7bcc7444ccf8e5b9-partinfo-00000000-QINU UNIQ7bcc7444ccf8e5b9-partinfo-00000001-QINU

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 ===Applications of BBa_K4461002===
-[[File:gDNA total.jpg|200px|thumb|left|	We cloned a larger fragment that included nirBDC promoter from E.coli K-12 MG1655 gDNA. It is about 446 bp.]]
-[[File:2nd total.jpg|200px|thumb|left|After adding suffix and prefix to the promoter, the nirBDC promoter part will be 357bp.]]
-We construct a device BBa_K4461501, with backbone pSB1C3. Then, we test the FP intensity and OD for it and the graph below is the result.
-[[File: NirBDC-mCerulean.png |200px|thumb|left|This graph shows that the mCerulean expressed since the sixth hour.]]
+[[File:gDNA total.jpg|400px|thumb|center|Fig.1	We cloned a larger fragment that included nirBDC promoter from E.coli K-12 MG1655 gDNA. It is about 446 bp.]]
+[[File:2nd total.jpg|400px|thumb|center|Fig.2  After adding suffix and prefix to the promoter, the nirBDC promoter part will be 357bp.]]
+We construct a device BBa_K4461501, with backbone pSB1C3. Then, we test the FP intensity and OD for it and Fig.3 is the result.
+[[File: NirBDC-mCerulean.png |400px|thumb|center|Fig.3  This graph shows that the mCerulean expressed since the sixth hour.]]
 ===User Reviews===