Difference between revisions of "Part:BBa K4463009"
 
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This part can realize the integration of adsorption of heavy ions and precious metal ion, such as Cd(II), Cu(II) and Ag(I), which is aimed to apply in real industrial wastewater containing multiple metal ions. The composite part is consist of four basic parts, which are CsgA, GS-linker, ShMT3 and MBP3. CsgA is the major curli subunit protein of E.coli, which realize the surface display of ShMT3 and MBP3. ShMT3 and MBP3 are new basic parts by IGEM_XJTLU 2023, which are proteins function on the metal ion adsorption. For more information on detailed ShMT3(<a href="https://parts.igem.org/Part:BBa_K4463005">BBa_K4463005</a>) and MBP3(<a href="https://parts.igem.org/Part:BBa_K4463000
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This part can realize the integration of adsorption of heavy ions and precious metal ion, such as Cd(II), Cu(II) and Ag(I), which is aimed to apply in real industrial wastewater containing multiple metal ions. The composite part is consist of four basic parts, which are CsgA, GS-linker, ShMT3 and MBP3. CsgA is the major curli subunit protein of E.coli, which realize the surface display of ShMT3 and MBP3. ShMT3 and MBP3 are new basic parts created by IGEM_XJTLU 2022, which are proteins function on the metal ion adsorption. For more information on detailed ShMT3(<a href="https://parts.igem.org/Part:BBa_K4463005">BBa_K4463005</a>) and MBP3(<a href="https://parts.igem.org/Part:BBa_K4463000
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">BBa_K4463000</a>) please visit their section page.
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">BBa_K4463000</a>) please visit their part page.
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XITLU_China 2022 predicted the structure of this fusion protein and its binding sites of multiple metal ions, including Cu(II), Zn(II), Cd(II) and Ag(I).
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<img src="https://static.igem.wiki/teams/4463/wiki/figures-for-engineering-and-parts/1.png
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Figure 1
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Figure 2
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In the <a href="https://2022.igem.wiki/xjtlu-china/model">Model session</a>, we proved the binding affinity of our protein design, as a crucial part of our heavy metal absorption system and relevant research community. View <a href="https://github.com/JiayiLi21/iGEM-XJTLU2022_Dry-Lab/tree/main/1">batch file</a>.
  
  
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Latest revision as of 12:01, 10 October 2022


CsgA-ShMT3-MBP3
This part can realize the integration of adsorption of heavy ions and precious metal ion, such as Cd(II), Cu(II) and Ag(I), which is aimed to apply in real industrial wastewater containing multiple metal ions. The composite part is consist of four basic parts, which are CsgA, GS-linker, ShMT3 and MBP3. CsgA is the major curli subunit protein of E.coli, which realize the surface display of ShMT3 and MBP3. ShMT3 and MBP3 are new basic parts created by IGEM_XJTLU 2022, which are proteins function on the metal ion adsorption. For more information on detailed ShMT3(BBa_K4463005) and MBP3(BBa_K4463000) please visit their part page.

XITLU_China 2022 predicted the structure of this fusion protein and its binding sites of multiple metal ions, including Cu(II), Zn(II), Cd(II) and Ag(I).


Figure 1


Figure 2

In the Model session, we proved the binding affinity of our protein design, as a crucial part of our heavy metal absorption system and relevant research community. View batch file.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]