Difference between revisions of "Part:BBa K4225021:Design"

 
 
(One intermediate revision by the same user not shown)
Line 4: Line 4:
  
 
<partinfo>BBa_K4225021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4225021 SequenceAndFeatures</partinfo>
 
  
 
===Design Notes===
 
===Design Notes===
x
+
Spacers are added between the basic parts and it is codon optimized for our chassis DH5&#945;.
 
+
 
+
  
 
===Source===
 
===Source===

Latest revision as of 11:58, 10 October 2022


Pc-rDAO-6xHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1106
    Illegal BamHI site found at 2034
    Illegal BamHI site found at 2288
    Illegal XhoI site found at 904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Spacers are added between the basic parts and it is codon optimized for our chassis DH5α.

Source

All parts are obtained from biobricks

References