Difference between revisions of "Part:BBa K4192022"

 
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It can discharge oxalate form thallus, so that the bacteria can adapt to the high concentration of oxalic acid environment. It is original from Fomitopsis palustris strain TYP6137, and we have tested it in E.coli and Pseudomonas fluorescens.
 
It can discharge oxalate form thallus, so that the bacteria can adapt to the high concentration of oxalic acid environment. It is original from Fomitopsis palustris strain TYP6137, and we have tested it in E.coli and Pseudomonas fluorescens.
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
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===Usage===
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Basic part <i>FpOAR</i>, encoding the efflux channel of oxalate.In our project, we used this part to efflux oxalic acid produced by engineering bacteria to achieve extracellular enrichment of oxalic acid. After exogenously adding calcium ions, calcium oxalate protective film was formed.
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===Biology===
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===Characterization===
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The part comes from <i>Fomitopsis palustris</i> originally, therefore we need to test if the gene FpOAR can work in prokaryotes as well
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 11:24, 10 October 2022


FpOAR, oxalate efflux channel protein

It can discharge oxalate form thallus, so that the bacteria can adapt to the high concentration of oxalic acid environment. It is original from Fomitopsis palustris strain TYP6137, and we have tested it in E.coli and Pseudomonas fluorescens.


Usage

Basic part FpOAR, encoding the efflux channel of oxalate.In our project, we used this part to efflux oxalic acid produced by engineering bacteria to achieve extracellular enrichment of oxalic acid. After exogenously adding calcium ions, calcium oxalate protective film was formed.

Biology

Characterization

The part comes from Fomitopsis palustris originally, therefore we need to test if the gene FpOAR can work in prokaryotes as well Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 158
    Illegal PstI site found at 301
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 158
    Illegal PstI site found at 301
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 158
    Illegal BglII site found at 1161
    Illegal XhoI site found at 973
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 158
    Illegal PstI site found at 301
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 158
    Illegal PstI site found at 301
  • 1000
    COMPATIBLE WITH RFC[1000]