Difference between revisions of "Part:BBa K4229013"
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TnaA codes for a tryptophanase. This tryptophanase turns L-tryptophan into either Indol. This biobrick consists of a version of TnaA, which is fused with the snoopcatcher(BBa_K4229009) on the N-terminal of the protein. With that, we made it able to be recruted into the wiffelball we used in our project. | TnaA codes for a tryptophanase. This tryptophanase turns L-tryptophan into either Indol. This biobrick consists of a version of TnaA, which is fused with the snoopcatcher(BBa_K4229009) on the N-terminal of the protein. With that, we made it able to be recruted into the wiffelball we used in our project. | ||
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Revision as of 10:54, 10 October 2022
TnaA with N-terminal SnoopCatcher
TnaA codes for a tryptophanase. This tryptophanase turns L-tryptophan into either Indol. This biobrick consists of a version of TnaA, which is fused with the snoopcatcher(BBa_K4229009) on the N-terminal of the protein. With that, we made it able to be recruted into the wiffelball we used in our project.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 977
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 977
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 977
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 977
Illegal AgeI site found at 142
Illegal AgeI site found at 228
Illegal AgeI site found at 1600 - 1000COMPATIBLE WITH RFC[1000]