Difference between revisions of "Part:BBa K215250"
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<partinfo>BBa_K215250 short</partinfo> | <partinfo>BBa_K215250 short</partinfo> | ||
− | This is a fusion protein that displays your favorite gene inserted into the NheI site. | + | This is a fusion protein that displays your favorite gene inserted into the NheI site. A TEV site is placed before the NheI site to allow for easy removal of the protein of interest from the surface of the cells by treatment with TEV protease. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 19:02, 1 October 2009
Lpp + 1tmr OmpA + GS Linker + TEV site + NheI Site
This is a fusion protein that displays your favorite gene inserted into the NheI site. A TEV site is placed before the NheI site to allow for easy removal of the protein of interest from the surface of the cells by treatment with TEV protease.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 211
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]