Difference between revisions of "Part:BBa K215107"

Revision as of 18:41, 1 October 2009

Extracellular Secretion System

This part is made of 3 genes: prtD, prtE, and prtF, that constitute a type I Erwinia chrysanthemi secretion system. The operon is expressed from a strong constitutive promoter, BBa_J23100, and has the translational terminator BBa_B0014. In PSB3T5.

Used to secrete proteins containing prtB C-terminal tag. The prtB C-terminal tag is built into the protein generator [BBa_K215002]. Any protein of interest can be inserted into the protein generator and then secreted when used in conjunction with this secretion system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1487
Illegal BsaI.rc site found at 1133
Illegal BsaI.rc site found at 3737

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K215107 short</partinfo>
-This is the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. The operon in under expression by a strong constitutive promoter, BBa_J23100, and has the translational terminator BBa_B0014. In PSB3T5
+This part is made of 3 genes: ''prtD, prtE'', and ''prtF'', that constitute a type I ''Erwinia chrysanthemi'' secretion system.  The operon is expressed from a strong constitutive promoter, BBa_J23100, and has the translational terminator BBa_B0014. In PSB3T5.
-Used to secrete proteins containing prtB C-terminus tag.  The prtB C-terminus tag is built into the protein generator  [[https://parts.igem.org/Part:BBa_K215002 BBa_K215002]].  Any protein of interest can be inserted into the protein generator and then secreted when used in conjunction with this secretion system.
+Used to secrete proteins containing prtB C-terminal tag.  The prtB C-terminal tag is built into the protein generator  [[https://parts.igem.org/Part:BBa_K215002 BBa_K215002]].  Any protein of interest can be inserted into the protein generator and then secreted when used in conjunction with this secretion system.
 <!-- Add more about the biology of this part here