Difference between revisions of "Part:BBa K215000"
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<partinfo>BBa_K215000 short</partinfo> | <partinfo>BBa_K215000 short</partinfo> | ||
− | This part contains the strong, IPTG-inducible promoter R0011, combined with the strong RBS B0034. This part can be used as a strong protein expression system when combined with a protein coding sequence. This part is in pSB1A3, which has a built in terminator | + | This part contains the strong, IPTG-inducible promoter R0011, combined with the strong RBS B0034. This part can be used as a strong protein expression system when combined with a protein coding sequence. This part is in pSB1A3, which has a built in transcriptional terminator located 3' of the insertion site for coding sequences. This allows for a protein coding sequence to be inserted into this vector and be immediately ready for expression. |
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Revision as of 18:17, 1 October 2009
R0011+B0034, strong IPTG-inducible promoter with strong RBS.
This part contains the strong, IPTG-inducible promoter R0011, combined with the strong RBS B0034. This part can be used as a strong protein expression system when combined with a protein coding sequence. This part is in pSB1A3, which has a built in transcriptional terminator located 3' of the insertion site for coding sequences. This allows for a protein coding sequence to be inserted into this vector and be immediately ready for expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]