Difference between revisions of "Part:BBa K3257005"

 
 
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==Added by LZU-HS-Pro-B==
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===Build Stage===
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We obtained the gene fragments we designed through DNA synthesis.
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-First, the plasmid was extracted for PCR amplification.  Then double digestion of PCR fragments and plasmids, nucleic acid electrophoresis, and gel recovery experiment were carried out.
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-The PCR fragments and plasmids digested successfully were recovered.  The target gene and plasmid were linked to transforming the linked product.
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-After the screening, the plasmid was successfully constructed.
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-Prepare receptor cells, and transform the engineering vector into the expression host E.coli BL21.
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===Test Stage===
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The red line in the middle shows the effect of the LacUV5 promoter ligating the red fluorescent protein.
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For the experimental results, it was evidence presented that the perceptive rate of uric acid greatly increased after we added the amplification system into our experiment.  We have discovered and concluded the general rule that the amplification system we designed and added was able to amplify the signal by a factor of four, which enabled us to better sense and detect the amount of uric acid in our bodies.
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[[Image: T--LZU-HS-Pro-B--Pro-B1.jpg | thumb | center | 500px ]]
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K3257005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3257005 SequenceAndFeatures</partinfo>
  

Latest revision as of 10:11, 10 October 2022

Added by LZU-HS-Pro-B

Build Stage

We obtained the gene fragments we designed through DNA synthesis.

-First, the plasmid was extracted for PCR amplification. Then double digestion of PCR fragments and plasmids, nucleic acid electrophoresis, and gel recovery experiment were carried out.

-The PCR fragments and plasmids digested successfully were recovered. The target gene and plasmid were linked to transforming the linked product.

-After the screening, the plasmid was successfully constructed.

-Prepare receptor cells, and transform the engineering vector into the expression host E.coli BL21.

Test Stage

The red line in the middle shows the effect of the LacUV5 promoter ligating the red fluorescent protein.

For the experimental results, it was evidence presented that the perceptive rate of uric acid greatly increased after we added the amplification system into our experiment. We have discovered and concluded the general rule that the amplification system we designed and added was able to amplify the signal by a factor of four, which enabled us to better sense and detect the amount of uric acid in our bodies.

T--LZU-HS-Pro-B--Pro-B1.jpg


LacUV5 Promoter

E.coli lac promoter with an /"up/" mutation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]