Difference between revisions of "Part:BBa K4463005"

 
 
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<partinfo>BBa_K4463005 short</partinfo>
 
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This is a improved part based on BBa_k4463001. The ability of Metallothioneins to bind metal ions depends on the amount of cysteine (Cys). Therefore, in order to improve the metal ion binding capacity of ShMT, we performed a three-point mutation of the original sequence by targeted mutagenesis to increase the Cys content.
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This is an improved part based on <a href="https://parts.igem.org/Part:BBa_k4463001">BBa_k4463001</a>.
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The ability of Metallothioneins to bind metal ions depends on the amount of cysteine (Cys). Therefore, in order to improve the metal ion binding capacity of ShMT, we performed a three-point mutation of the original sequence by targeted mutagenesis to increase the Cys content<sup>[1]</sup>.
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IGEM_XJTLU 2022 tested the function of ShMT3 by adding it with CsgA, which realized the surface display of ShMT3 on <i>E.coli</i>.
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<img src="https://static.igem.wiki/teams/4463/wiki/figures-for-engineering-and-parts/shmt-adsorb-cu-1800.png
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" style="width:40%;height=40%; "></img>
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Figure1. <i>E.coli</i> expressed ShMT3 protein showed higher adsorption of Cu(II) in 1.8mM CuSO4 solution.
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<img src="https://static.igem.wiki/teams/4463/wiki/figures-for-engineering-and-parts/shmt-adsorb-cd1.png
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Figure2. <i>E.coli</i> expressed ShMT3 protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.
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We used ICP-MS to measure the concentration of binding Cu(II) and Cd(II) on the surface of <i>E.coli</i>. For detailed information about our measurement method and result analysis, please visit our <a href="https://2022.igem.wiki/xjtlu-china/experiments">Experiments</a>and <a href="https://2022.igem.wiki/xjtlu-china/results">Results</a>on wiki.
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<h3>Reference </h3>
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1 X. Li, Z. Ren, M. J. C. Crabbe, L. Wang and W. Ma, <i>Ecotoxicology and Environmental Safety</i>, 2021, 222, 112512.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 09:46, 10 October 2022


ShMT3


This is an improved part based on BBa_k4463001. The ability of Metallothioneins to bind metal ions depends on the amount of cysteine (Cys). Therefore, in order to improve the metal ion binding capacity of ShMT, we performed a three-point mutation of the original sequence by targeted mutagenesis to increase the Cys content[1].

IGEM_XJTLU 2022 tested the function of ShMT3 by adding it with CsgA, which realized the surface display of ShMT3 on E.coli.

Figure1. E.coli expressed ShMT3 protein showed higher adsorption of Cu(II) in 1.8mM CuSO4 solution.
Figure2. E.coli expressed ShMT3 protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.

We used ICP-MS to measure the concentration of binding Cu(II) and Cd(II) on the surface of E.coli. For detailed information about our measurement method and result analysis, please visit our Experimentsand Resultson wiki.

Reference

1 X. Li, Z. Ren, M. J. C. Crabbe, L. Wang and W. Ma, Ecotoxicology and Environmental Safety, 2021, 222, 112512.  



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]