Difference between revisions of "Part:BBa K4375014"
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==Usage and Biology== | ==Usage and Biology== | ||
− | The Myc-tag is derived from the human c-myc oncogene | + | The Myc-tag, a commonly used tag in research and biological applications, is derived from the human c-myc oncogene (410-419 at the C-terminus). The Myc-tag is used in detecting the expression of recombinant proteins when it is fused to the protein of interest. Its sequence is EQKLISEEDL. It is commonly used in Western blotting, ELISA, protein purification ot co-immunoprecipitation. |
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<partinfo>BBa_K4375014 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4375014 SequenceAndFeatures</partinfo> | ||
− | == | + | ==Reference== |
− | https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/ | + | „Myc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification.” https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/ |
Revision as of 09:31, 10 October 2022
Myc Tag
Myc Tag derived from c-Myc.
Usage and Biology
The Myc-tag, a commonly used tag in research and biological applications, is derived from the human c-myc oncogene (410-419 at the C-terminus). The Myc-tag is used in detecting the expression of recombinant proteins when it is fused to the protein of interest. Its sequence is EQKLISEEDL. It is commonly used in Western blotting, ELISA, protein purification ot co-immunoprecipitation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
„Myc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification.” https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/