Difference between revisions of "Part:BBa K4375011"
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<partinfo>BBa_K4375011 short</partinfo>
 
<partinfo>BBa_K4375011 short</partinfo>
  
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It is a natural llama hinge region to provide rigid linker for Nanobody and the protein fused with it. Sequence is: STPPTPSPSTPP.
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It is a natural llama hinge region to provide a flexible linker for Nanobody and the protein fused to it. The amino acid sequence is STPPTPSPSTPP.
  
 
==Usage and Biology==
 
==Usage and Biology==
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<partinfo>BBa_K4375011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4375011 SequenceAndFeatures</partinfo>
  
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==References==
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==Reference==
  
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760621/
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Li D, Ren J, Ji F, Peng Q, Teng H, Jia L. Peptide linker affecting the activity retention rate of VHH in immunosorbents. Biomolecules. 2020 Nov 27;10(12):1610.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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Revision as of 09:20, 10 October 2022


Llama immunoglobulin IgA hinge linker

It is a natural llama hinge region to provide a flexible linker for Nanobody and the protein fused to it. The amino acid sequence is STPPTPSPSTPP.

Usage and Biology

This region in the antibody ensures that the variable regions can move flexibly in space and thus bind to their antigen. Since it fulfills this role in nature, it is suitable for conjugation to nanobodies. This way the Nanobody remains flexible and can attach to the chosen antigen after conjugation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

Li D, Ren J, Ji F, Peng Q, Teng H, Jia L. Peptide linker affecting the activity retention rate of VHH in immunosorbents. Biomolecules. 2020 Nov 27;10(12):1610.