Difference between revisions of "Part:BBa K4361012"
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<partinfo>BBa_K4361012 short</partinfo> | <partinfo>BBa_K4361012 short</partinfo> | ||
| − | + | BlcR is a transcription factor originating from the bacterium <i>Agrobacterium tumefaciens</i> ([[Part:BBa_K4361100]]). In a homodimer state it contains a single DNA-binding domain that specifically binds one of two DNA sequences. Both sequences are so-called inverted repeat pairs (IRs), short DNA sequences whose ends are reverse complements of each other. For the Blc operator, these sequences are 'ACTCTAATgATTCAAGT' (IR1) and 'ATTAGttgaactCTAAT' (IR2), as further explained in [[Part:BBa_K4361001]]. <br> | |
| + | [[Part:BBa_K4361010]] up to [[Part:BBa_K4361013]] have been designed to test whether or not the exact orientation of IRs influences the binding strength of BlcR to DNA. This is achieved by 'flipping' the IRs, or replacing them by their reverse complements. To create this part, the nucleotides originally designated as belonging to IR1 have been replaced by the reverse complement of IR1 and those of IR2 by the reverse complement of IR2, resulting in 'ACTTGAATcATTAGAGT' (IR1 flip) 'ATTAGagttcaaCTAAT' (IR2 flip). The BlcR-binding domain of this part thus consists of IR1 flip-tca-IR2 flip, where tca is the original 3 nt linker sequence between IRs. | ||
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Revision as of 08:01, 10 October 2022
BlcR-binding oligo, 51 bp, IR1 flip + IR2 flip
BlcR is a transcription factor originating from the bacterium Agrobacterium tumefaciens (Part:BBa_K4361100). In a homodimer state it contains a single DNA-binding domain that specifically binds one of two DNA sequences. Both sequences are so-called inverted repeat pairs (IRs), short DNA sequences whose ends are reverse complements of each other. For the Blc operator, these sequences are 'ACTCTAATgATTCAAGT' (IR1) and 'ATTAGttgaactCTAAT' (IR2), as further explained in Part:BBa_K4361001.
Part:BBa_K4361010 up to Part:BBa_K4361013 have been designed to test whether or not the exact orientation of IRs influences the binding strength of BlcR to DNA. This is achieved by 'flipping' the IRs, or replacing them by their reverse complements. To create this part, the nucleotides originally designated as belonging to IR1 have been replaced by the reverse complement of IR1 and those of IR2 by the reverse complement of IR2, resulting in 'ACTTGAATcATTAGAGT' (IR1 flip) 'ATTAGagttcaaCTAAT' (IR2 flip). The BlcR-binding domain of this part thus consists of IR1 flip-tca-IR2 flip, where tca is the original 3 nt linker sequence between IRs.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and biology
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Results
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