Difference between revisions of "Part:BBa K4325026"
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===Description=== | ===Description=== | ||
This composite part is a generator containing pDawn (CI-LVA) and LKD. | This composite part is a generator containing pDawn (CI-LVA) and LKD. | ||
| + | ===Biology=== | ||
| + | This composite part is a generator containing pDawn (CI-LVA) (<partinfo>BBa_K1075044</partinfo>) and LKD (<partinfo>BBa_K4325004</partinfo>). | ||
| Line 15: | Line 17: | ||
<partinfo>BBa_K4325026 parameters</partinfo> | <partinfo>BBa_K4325026 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
| + | ===2022 SZPT-China= | ||
| + | <h3>Biology</h3> | ||
| + | <p>This composite part is a generator containing pDawn (CI-LVA) (<partinfo>BBa_K1075044</partinfo>) and LKD (<partinfo>BBa_K4325004</partinfo>).</p> | ||
| + | <h3>Characterization</h3> | ||
| + | <h4>Characterization in <i>E. coli</i> TOP10</h4> | ||
| + | <p>As shown in Figure1, 14 colonies meet our expectation and one of them was picked and culture in the dark and under blue light. Growth curves shows the bacteria continue growing after 8 hours, indicating this system is not robust.</p> | ||
| + | [[File:K26 1.png|600px|thumb|center|Figure 1: <p></p>(a) Colony growth of <i>E. coli</i> TOP10 containing pSEVA331-pDawn(cI-LVA)-RBS070-LKD-T1 in the dark <p></p> | ||
| + | (a2) Colony growth of <i>E. coli</i> TOP10 containing pSEVA331-pDawn(cI-LVA)-RBS070-LKD-T1 under blue light <p></p> | ||
| + | (a3) Colony growth of <i>E. coli</i> TOP10-pSEVA331-pDawn(cI-LVA)-RBS070-LKD-T1;]] | ||
| + | <h4>Characterization in <i>G. hansenii</i> ATCC53582</h4> | ||
| + | <p>The picked plasmids from <i> E. coli</i> TOP10 were transformed into <i> G. hansenii</i> ATCC53582. It shows that these two plasmids still maintain their excellent lysis effect in <i>G. hansenii</i> ATCC53582.</p> | ||
| + | [[File:K26 2.png|600px|thumb|center|Figure 2: <p></p>(a1) Colony growth of <i>G. hansenii</i> ATCC53582 containing pSEVA331-pDawn(cI-LVA)-RBS070-LKD-T1 in the dark <p></p> | ||
| + | (a2) Colony growth of <i>G. hansenii</i> ATCC53582 containing pSEVA331-pDawn(cI-LVA)-RBS070-LKD-T1 under blue light]] | ||
Revision as of 06:07, 10 October 2022
pDawn-RBS070-LKD-T1
Description
This composite part is a generator containing pDawn (CI-LVA) and LKD.
Biology
This composite part is a generator containing pDawn (CI-LVA) (BBa_K1075044) and LKD (BBa_K4325004).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2171
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 63
Illegal NgoMIV site found at 195
Illegal NgoMIV site found at 289
Illegal NgoMIV site found at 582
Illegal NgoMIV site found at 1076
Illegal NgoMIV site found at 1094
Illegal NgoMIV site found at 1184
Illegal AgeI site found at 414
Illegal AgeI site found at 1542 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1643
Illegal BsaI.rc site found at 525
==2022 SZPT-China
Biology
This composite part is a generator containing pDawn (CI-LVA) (BBa_K1075044) and LKD (BBa_K4325004).
Characterization
Characterization in E. coli TOP10
As shown in Figure1, 14 colonies meet our expectation and one of them was picked and culture in the dark and under blue light. Growth curves shows the bacteria continue growing after 8 hours, indicating this system is not robust.
Characterization in G. hansenii ATCC53582
The picked plasmids from E. coli TOP10 were transformed into G. hansenii ATCC53582. It shows that these two plasmids still maintain their excellent lysis effect in G. hansenii ATCC53582.
