Difference between revisions of "Part:BBa K4169006"
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<p>Under natural conditions, some RNA with special structures can form stem loops autonomously. Complete with RNA Thermometer(HZAU-China 2021:BBa_K3733010) was used in this study.</p> | <p>Under natural conditions, some RNA with special structures can form stem loops autonomously. Complete with RNA Thermometer(HZAU-China 2021:BBa_K3733010) was used in this study.</p> | ||
Revision as of 05:33, 10 October 2022
HepT: a RNase like toxin
Under natural conditions, some RNA with special structures can form stem loops autonomously. Complete with RNA Thermometer(HZAU-China 2021:BBa_K3733010) was used in this study.
Usage and Biology
The RNA thermometer has a special sequence capable of forming stem-loop structures at 27 ° C and below and opening at 37 ° C. At the same time, there is a specific binding site for RNase E on the sequence that can form the secondary structure. At the transcriptional level, if this sequence is present on mRNA, it is able to bind RNase E and thus degrade the entire RNA strand. Therefore, we introduce the thermometer into the suicide module to achieve temperature sensitive suicide.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 103
Illegal PstI site found at 127 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 103
Illegal PstI site found at 127 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 103
Illegal PstI site found at 127 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 103
Illegal PstI site found at 127 - 1000COMPATIBLE WITH RFC[1000]
References
Hoynes-O'Connor A, Hinman K, Kirchner L, Moon TS. De novo design of heat-repressible RNA thermosensors in E. coli. Nucleic Acids Res. 2015 Jul 13;43(12):6166-79.