Difference between revisions of "Part:BBa K4169006"

 
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===RNA Thermometer===
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<p>Under natural conditions, some RNA with special structures can form stem loops autonomously. Complete with RNA Thermometer(HZAU-China 2021:BBa_K3733010) was used in this study.</p>
  
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HepT, a member of the higher eukaryotes and prokaryotes nucleotide-binding (HEPN) superfamily, strongly inhibiting cell growth in S.oneidensis and Escherichia coli. HepT can bind to mRNA in cells and induce RNA degradation. HepT dimerization enables the formation of a deep cleft at the HEPN-domain interface harboring a composite Rx4-6H active site that functions as an RNA-cleaving ribonuclease.
 
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===Usage and Biology===
 
===Usage and Biology===
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<p>The RNA thermometer has a special sequence capable of forming stem-loop structures at 27 ° C and below and opening at 37 ° C. At the same time, there is a specific binding site for RNase E on the sequence that can form the secondary structure. At the transcriptional level, if this sequence is present on mRNA, it is able to bind RNase E and thus degrade the entire RNA strand. Therefore, we introduce the thermometer into the suicide module to achieve temperature sensitive suicide.
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4169006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4169006 SequenceAndFeatures</partinfo>
  
  
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===Functional Parameters===
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<h3>References</h3>
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<partinfo>BBa_K4169006 parameters</partinfo>
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<p>Hoynes-O'Connor A, Hinman K, Kirchner L, Moon TS. De novo design of heat-repressible RNA thermosensors in E. coli.<i> Nucleic Acids Res.</i> 2015 Jul 13;43(12):6166-79.</p>
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Revision as of 05:30, 10 October 2022


BBa_K4169006

RNA Thermometer

Under natural conditions, some RNA with special structures can form stem loops autonomously. Complete with RNA Thermometer(HZAU-China 2021:BBa_K3733010) was used in this study.

Usage and Biology

The RNA thermometer has a special sequence capable of forming stem-loop structures at 27 ° C and below and opening at 37 ° C. At the same time, there is a specific binding site for RNase E on the sequence that can form the secondary structure. At the transcriptional level, if this sequence is present on mRNA, it is able to bind RNase E and thus degrade the entire RNA strand. Therefore, we introduce the thermometer into the suicide module to achieve temperature sensitive suicide.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 103
    Illegal PstI site found at 127
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 103
    Illegal PstI site found at 127
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 103
    Illegal PstI site found at 127
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 103
    Illegal PstI site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Hoynes-O'Connor A, Hinman K, Kirchner L, Moon TS. De novo design of heat-repressible RNA thermosensors in E. coli. Nucleic Acids Res. 2015 Jul 13;43(12):6166-79.