Difference between revisions of "Part:BBa K215107"

Revision as of 04:36, 1 October 2009

Extracellular Secretion System

This is the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. The operon in under expression by a strong constitutive promoter, BBa_J23100, and has the translational terminator BBa_B0014. In PSB3T5

Used to secrete proteins containing prtB C-terminus tag. The prtB C-terminus tag is built into the protein generator [BBa_K215002]. Any protein of interest can be inserted into the protein generator and then secreted when used in conjunction with this secretion system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1487
Illegal BsaI.rc site found at 1133
Illegal BsaI.rc site found at 3737

@@ Line 4: / Line 4: @@
 This is the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. The operon in under expression by a strong constitutive promoter, BBa_J23100, and has the translational terminator BBa_B0014. In PSB3T5
-Used to secrete proteins containing prtB C-terminus tag, expression vector [[https://parts.igem.org/Part:BBa_K215002 BBa_K215002]] should be used.
+Used to secrete proteins containing prtB C-terminus tag.  The prtB C-terminus tag is built into the protein generator  [[https://parts.igem.org/Part:BBa_K215002 BBa_K215002]].  Any protein of interest can be inserted into the protein generator and then secreted when used in conjunction with this secretion system.
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