Difference between revisions of "Part:BBa K4452017"

 
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<partinfo>BBa_K4452017 short</partinfo>
 
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RUBY reporter under 35S promoter
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For visual selection of positively transformed seeds, RUBY reporter is expressed with the constitutive CMV35S plant specific promoter, a 5’ UTR from the AtRbcS2B gene, and a 3’ UTR plant terminator. The RUBY reporter construct will make the seeds red from the expression of betalain pigments
  
 
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Latest revision as of 04:36, 10 October 2022


RUBY reporter under 35S promoter

For visual selection of positively transformed seeds, RUBY reporter is expressed with the constitutive CMV35S plant specific promoter, a 5’ UTR from the AtRbcS2B gene, and a 3’ UTR plant terminator. The RUBY reporter construct will make the seeds red from the expression of betalain pigments

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 226
    Illegal BglII site found at 4234
    Illegal BamHI site found at 2883
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 819
    Illegal NgoMIV site found at 1398
    Illegal NgoMIV site found at 3111
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4629
    Illegal BsaI.rc site found at 4908