Difference between revisions of "Part:BBa K215200:Design"
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===Design Notes=== | ===Design Notes=== | ||
We took the Lpp Signaling Peptide and OmpA 1 trans-membrane region from Havard 2006, BBa_J36848, with two custom primers: | We took the Lpp Signaling Peptide and OmpA 1 trans-membrane region from Havard 2006, BBa_J36848, with two custom primers: | ||
| − | Lpp Forward: 5'-gcggccgctTCTAGAtgaaagctactaaactggtactggg-3' | + | * Lpp Forward: 5'-gcggccgctTCTAGAtgaaagctactaaactggtactggg-3' |
| − | OmpA Reverse with GS Linker, GGGSGGGSGGG, and a TEV site, ENLYFQG, and SpeI site: 5'-cggccgctACTAGTaGCTAGCaccctgaaaatacaggttttcACCACCACCAGAACCACCACCAGAACCACCACCgctgcctttgtacggcatacgacc-3' | + | * OmpA Reverse with GS Linker, GGGSGGGSGGG, and a TEV site, ENLYFQG, and SpeI site: 5'-cggccgctACTAGTaGCTAGCaccctgaaaatacaggttttcACCACCACCAGAACCACCACCAGAACCACCACCgctgcctttgtacggcatacgacc-3' |
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===Source=== | ===Source=== | ||
Latest revision as of 03:49, 1 October 2009
Generic Surface Display Construct: IPTG Inducible Lpp + 1tmr OmpA + NheI Site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 292
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We took the Lpp Signaling Peptide and OmpA 1 trans-membrane region from Havard 2006, BBa_J36848, with two custom primers:
- Lpp Forward: 5'-gcggccgctTCTAGAtgaaagctactaaactggtactggg-3'
- OmpA Reverse with GS Linker, GGGSGGGSGGG, and a TEV site, ENLYFQG, and SpeI site: 5'-cggccgctACTAGTaGCTAGCaccctgaaaatacaggttttcACCACCACCAGAACCACCACCAGAACCACCACCgctgcctttgtacggcatacgacc-3'
Source
The Lpp + 1tmr OmpA is from Havard iGEM 2006: BBa_J36848, which is from [cite].