Difference between revisions of "Part:BBa K3748011"
(→‎Team Estonia_TUIT characterization of BBa_K3748015 (pPAB1))
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==Team Estonia_TUIT characterization of BBa_K3748015 (<i>pPAB1</i>)==
 
==Team Estonia_TUIT characterization of BBa_K3748015 (<i>pPAB1</i>)==
  
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Constitutive <i>pPAB1</i> promoter (Michael E. Lee et. al, 2015), controls a gene encoding the <i>Pab1<i> protein. This protein binds Poly(A) and is involved in the control of poly(A) tail length on nuclear mRNA transcripts (N Amrani et. al, 1997).
+
Constitutive <i>pPAB1</i> promoter (Michael E. Lee et. al, 2015), controls a gene encoding the <i>Pab1</i> protein. This protein binds Poly(A) and is involved in the control of poly(A) tail length on nuclear mRNA transcripts (N Amrani et. al, 1997).
  
 
<i>pPAB1</i> promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, <i>pPAB1</i> showed a 19-fold increase in fluorescence intensity (figure 1).
 
<i>pPAB1</i> promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, <i>pPAB1</i> showed a 19-fold increase in fluorescence intensity (figure 1).

Revision as of 22:14, 9 October 2022


pPAB1

S.cerevisiae medium strenght promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Team Estonia_TUIT characterization of BBa_K3748015 (pPAB1)

Constitutive pPAB1 promoter (Michael E. Lee et. al, 2015), controls a gene encoding the Pab1 protein. This protein binds Poly(A) and is involved in the control of poly(A) tail length on nuclear mRNA transcripts (N Amrani et. al, 1997).

pPAB1 promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, pPAB1 showed a 19-fold increase in fluorescence intensity (figure 1).

Figure 1: Graph depicting median fluorescence intensity of pPAB1 and control strain

References:

  • A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. Michael E. Lee, William C. DeLoach, Bernardo Cervantes, and John E. Dueber, 2015
  • Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro, N Amrani, M Minet, M Le Gouar, F Lacroute, F Wyers, 1997