Difference between revisions of "Part:BBa K3748015"
Kiselev sl (Talk | contribs) (→Team Estonia_TUIT characterization of BBa_K3748015 (pREV1)) |
Kiselev sl (Talk | contribs) (→Team Estonia_TUIT characterization of BBa_K3748015 (pREV1)) |
||
Line 22: | Line 22: | ||
<i>pREV1</i> is a constitutive promoter (Michael E. Lee et. al, 2015) responsible for the expression of <i>Rev1</i>, a gene encoding bi-functional DNA-directed DNA polymerase/deoxycytidyl transferase. This enzyme is involved in error-prone translesion synthesis, one of the pathways for DNA repair (Lawrence CW, 2004). | <i>pREV1</i> is a constitutive promoter (Michael E. Lee et. al, 2015) responsible for the expression of <i>Rev1</i>, a gene encoding bi-functional DNA-directed DNA polymerase/deoxycytidyl transferase. This enzyme is involved in error-prone translesion synthesis, one of the pathways for DNA repair (Lawrence CW, 2004). | ||
− | <i>pREV1</i> promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, <i>pREV1</i> demonstrated a five-fold increase in fluorescence intensity | + | <i>pREV1</i> promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, <i>pREV1</i> demonstrated a five-fold increase in fluorescence intensity (figure 1). |
Revision as of 20:51, 9 October 2022
pREV1
Weak strenght yeast promoter S.cerevisiae.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 705
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Team Estonia_TUIT characterization of BBa_K3748015 (pREV1)
pREV1 is a constitutive promoter (Michael E. Lee et. al, 2015) responsible for the expression of Rev1, a gene encoding bi-functional DNA-directed DNA polymerase/deoxycytidyl transferase. This enzyme is involved in error-prone translesion synthesis, one of the pathways for DNA repair (Lawrence CW, 2004).
pREV1 promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, pREV1 demonstrated a five-fold increase in fluorescence intensity (figure 1).
References:
- A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. Michael E. Lee, William C. DeLoach, Bernardo Cervantes, and John E. Dueber, 2015
- Cellular functions of DNA polymerase zeta and Rev1 protein, Lawrence CW (2004), Adv Protein Chem