Difference between revisions of "Part:BBa K4268014"

 
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<partinfo>BBa_K4268014 short</partinfo>
 
<partinfo>BBa_K4268014 short</partinfo>
  
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This part classifies as Level 1 Transcriptional Unit of Golden Gate Assembly. It contains a promoter + RBS, coding sequence and terminator. The promoter and RBS used was  BBa_R0085 + BBa_B0034. The terminator used was BBa_B0015.  
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===Usage and Biology===
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S-TIP37 is a T7-like cyanophage that infects its host via a lytic life cycle (Shitrit et al., 2021). T7-like phages are characterized by a complex symmetrical capsid structure, which includes an icosahedral head that houses the phage's genome, and an internal core region that stabilizes the packaged DNA inside the head. A neck region that facilitates DNA delivery into a host, and six tail fibers used for attachment to its host (Raytcheva et al., 2011).
  
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[[File:T--Suny Oneonta-t7-virus-structure.jpg|200px|frame|center|Figure 1: A labeled visual detailing the various structures of a T7-like phage (Kemp et al., 2005)]]
  
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This part belongs to a collection that codes for a "ghost" phage. This ghost phage is a capsid-only, empty viral shell that could be modified to immobilize Cyanobacteria recognized by the viral tail fibers, or used with modification to deliver substances to a chassis Cyanobacteria.
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===Usage and Biology===
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However, it will infect ''Synechococcus sp WH 8109'', the cyanobacteria strain that is the natural host of S-TIP37. Further modeling will be needed to determine if the "ghost" phage could effectively target other strains of Cyanobacteria that are used in synthetic biology, such as ''Synechococcus sp PCC 11901''.
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The Internal Core Protein Transcriptional Unit classifies as a Level 1 Transcriptional Unit of Golden Gate Assembly. A strong promoter and RBS were used, BBa_R0085 + BBa_B0034. The terminator used was BBa_B0015.
  
 
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<partinfo>BBa_K4268014 parameters</partinfo>
 
<partinfo>BBa_K4268014 parameters</partinfo>
 
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===References===
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Kemp P, Garcia LR, Molineux IJ. Changes in bacteriophage T7 virion structure at the initiation of infection. Virology. 2005 Sep 30;340(2):307-17. doi: 10.1016/j.virol.2005.06.039. PMID: 16054667
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Raytcheva DA, Haase-Pettingell C, Piret JM, King JA. Intracellular assembly of cyanophage Syn5 proceeds through a scaffold-containing procapsid. J Virol. 2011 Mar;85(5):2406-15. doi: 10.1128/JVI.01601-10. Epub 2010 Dec 22. PMID: 21177804; PMCID: PMC3067778.
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Shitrit D, Hackl T, Laurenceau R, Raho N, Carlson MCG, Sabehi G, Schwartz DA, Chisholm SW, Lindell D. Genetic engineering of marine cyanophages reveals integration but not lysogeny in T7-like cyanophages. ISME J. 2022 Feb;16(2):488-499. doi: 10.1038/s41396-021-01085-8. Epub 2021 Aug 24. PMID: 34429521; PMCID: PMC8776855.

Latest revision as of 20:27, 9 October 2022


Internal Core Protein Transcriptional Unit

Usage and Biology

S-TIP37 is a T7-like cyanophage that infects its host via a lytic life cycle (Shitrit et al., 2021). T7-like phages are characterized by a complex symmetrical capsid structure, which includes an icosahedral head that houses the phage's genome, and an internal core region that stabilizes the packaged DNA inside the head. A neck region that facilitates DNA delivery into a host, and six tail fibers used for attachment to its host (Raytcheva et al., 2011).

Figure 1: A labeled visual detailing the various structures of a T7-like phage (Kemp et al., 2005)

This part belongs to a collection that codes for a "ghost" phage. This ghost phage is a capsid-only, empty viral shell that could be modified to immobilize Cyanobacteria recognized by the viral tail fibers, or used with modification to deliver substances to a chassis Cyanobacteria.

However, it will infect Synechococcus sp WH 8109, the cyanobacteria strain that is the natural host of S-TIP37. Further modeling will be needed to determine if the "ghost" phage could effectively target other strains of Cyanobacteria that are used in synthetic biology, such as Synechococcus sp PCC 11901.

The Internal Core Protein Transcriptional Unit classifies as a Level 1 Transcriptional Unit of Golden Gate Assembly. A strong promoter and RBS were used, BBa_R0085 + BBa_B0034. The terminator used was BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3443
    Illegal NgoMIV site found at 3582
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Kemp P, Garcia LR, Molineux IJ. Changes in bacteriophage T7 virion structure at the initiation of infection. Virology. 2005 Sep 30;340(2):307-17. doi: 10.1016/j.virol.2005.06.039. PMID: 16054667

Raytcheva DA, Haase-Pettingell C, Piret JM, King JA. Intracellular assembly of cyanophage Syn5 proceeds through a scaffold-containing procapsid. J Virol. 2011 Mar;85(5):2406-15. doi: 10.1128/JVI.01601-10. Epub 2010 Dec 22. PMID: 21177804; PMCID: PMC3067778.

Shitrit D, Hackl T, Laurenceau R, Raho N, Carlson MCG, Sabehi G, Schwartz DA, Chisholm SW, Lindell D. Genetic engineering of marine cyanophages reveals integration but not lysogeny in T7-like cyanophages. ISME J. 2022 Feb;16(2):488-499. doi: 10.1038/s41396-021-01085-8. Epub 2021 Aug 24. PMID: 34429521; PMCID: PMC8776855.