Difference between revisions of "Part:BBa K3748015"
Kiselev sl (Talk | contribs) (2022 Estonia_TUIT contribution) |
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<partinfo>BBa_K3748015 parameters</partinfo> | <partinfo>BBa_K3748015 parameters</partinfo> | ||
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| + | ==Team Estonia_TUIT characterization of BBa_K3748015 (<i>pREV1</i>)== | ||
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| + | <i>pREV1</i> is a constitutive promoter (Michael E. Lee et. al, 2015) responsible for the expression of <i>Rev1</i>, a gene encoding bi-functional DNA-directed DNA polymerase/deoxycytidyl transferase. This enzyme is involved in error-prone translesion synthesis, one of the pathways for DNA repair (Lawrence CW, 2004). | ||
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| + | <i>pREV1</i> promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, <i>pREV1</i> demonstrated a five-fold increase in fluorescence intensity, respectively (figure 1). The results confirm the data from Michael E. Lee’s article from 2015, where the <i>pREV1</i> was the weakest promoter present in the kit. | ||
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| + | [[Image:PREV1 Estonia TUIT.png.png|600px|thumb|center|'''Figure 1:''' <b>Graph depicting median fluorescence intensity of <i>pREV1</i> and control strain</b>]] | ||
Revision as of 20:07, 9 October 2022
pREV1
Weak strenght yeast promoter S.cerevisiae.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 705
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Team Estonia_TUIT characterization of BBa_K3748015 (pREV1)
pREV1 is a constitutive promoter (Michael E. Lee et. al, 2015) responsible for the expression of Rev1, a gene encoding bi-functional DNA-directed DNA polymerase/deoxycytidyl transferase. This enzyme is involved in error-prone translesion synthesis, one of the pathways for DNA repair (Lawrence CW, 2004).
pREV1 promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, pREV1 demonstrated a five-fold increase in fluorescence intensity, respectively (figure 1). The results confirm the data from Michael E. Lee’s article from 2015, where the pREV1 was the weakest promoter present in the kit.
