Difference between revisions of "Part:BBa K4165020"

(→‎Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
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His tag is used for purifying and detecting the recombinant protein without a protein-specific antibody or probe (1). All parts of our trim system are fused with his tag for purification.
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His tag is used for purifying and detecting the recombinant protein without a protein-specific antibody or probe (1). Parts of our systems are fused with his tag for purification and pull-down assay.
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===Source===
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(pGS-21a) vector, the backbone was retrieved from add gene.
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===References===
 
===References===

Revision as of 19:37, 9 October 2022


6x Histidine tag

This part encodes for 6x Histidine tag used for purification and characterization of proteins

Usage and Biology

His tag is used for purifying and detecting the recombinant protein without a protein-specific antibody or probe (1). Parts of our systems are fused with his tag for purification and pull-down assay.

References

1- Spriestersbach A, Kubicek J, Schäfer F, Block H, Maertens B. Purification of His-Tagged Proteins. Methods Enzymol. 2015;559:1-15. doi: 10.1016/bs.mie.2014.11.003. Epub 2015 May 4. PMID: 26096499.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]