Difference between revisions of "Part:BBa K4428001:Design"

 
 
(2 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Designed such that only the metal binding domain of the protein is obtained
+
Codon optimised for the chassis E. coli by changing the 150<sup>th</sup> nucleotide from T to C
−
 
+
−
 
+
  
 
===Source===
 
===Source===
Line 16: Line 14:
  
 
===References===
 
===References===
 +
 +
Hui, CY., Guo, Y., Yang, XQ. et al. Surface display of metal binding domain derived from PbrR on Escherichia coli specifically increases lead(II) adsorption. Biotechnol Lett 40, 837–845 (2018). https://doi.org/10.1007/s10529-018-2533-4

Latest revision as of 18:58, 9 October 2022


PbrR MBD - Lead-binding domain of PbrR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 229
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 229
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 229
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 20


Design Notes

Codon optimised for the chassis E. coli by changing the 150th nucleotide from T to C

Source

Truncated part of PbrR

References

Hui, CY., Guo, Y., Yang, XQ. et al. Surface display of metal binding domain derived from PbrR on Escherichia coli specifically increases lead(II) adsorption. Biotechnol Lett 40, 837–845 (2018). https://doi.org/10.1007/s10529-018-2533-4