Difference between revisions of "Part:BBa K200005"
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Sequence codes for trehalose-6-phosphate synthase enzyme. This enzyme is the first of two required in the conversion of glucose to trehalose. <br> | Sequence codes for trehalose-6-phosphate synthase enzyme. This enzyme is the first of two required in the conversion of glucose to trehalose. <br> | ||
| − | This enzyme catalyses the following reaction: <br> | + | This enzyme catalyses the following reaction: <br><br> |
| − | + | ||
| − | <br> | + | |
| − | [en.wikipedia.org/wiki/Trehalose Trehalose] is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock <cite>otsa1</cite>, and is naturally produced in Escherichia Coli. We hope that by upregulating the trehalose production pathways in E.coli we can increase trehalose concentrations within our cell, thereby conferring some resistance to protein degredation in our system. This would allow easy transport and storage of the final product.<br><br> | + | [[Image:II09_OtsA.png]] |
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| + | <br><br> | ||
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| + | [http://en.wikipedia.org/wiki/Trehalose Trehalose] is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock <cite>otsa1</cite>, and is naturally produced in Escherichia Coli. We hope that by upregulating the trehalose production pathways in E.coli we can increase trehalose concentrations within our cell, thereby conferring some resistance to protein degredation in our system. This would allow easy transport and storage of the final product.<br><br> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Transcription of OtsA gene is activated by osmotic stress in E. coli.<cite> | + | Transcription of OtsA gene is activated by osmotic stress in E. coli.<cite>otsa1</cite> |
| − | mRNA of OtsA is more stable at 16°C, therefore, it is a cold inducible mRNA.<cite> | + | mRNA of OtsA is more stable at 16°C, therefore, it is a cold inducible mRNA.<cite>otsa2</cite> |
| − | OtsA mutation will block the synthesis of the trehalose-6-phosphase synthase, which is the enzyme that converts glucose-6-phosphate to trehalose-6-phosphate. <cite> | + | OtsA mutation will block the synthesis of the trehalose-6-phosphase synthase, which is the enzyme that converts glucose-6-phosphate to trehalose-6-phosphate. <cite>otsa2</cite> |
| + | The gene was used alongside [[Part:BBa_K200006 |OtsB]] by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] team as part of the storage protection mechanism. It was also previously used by the UC Berkeley 2007 iGEM team as part of the [http://2007.igem.org/Berkeley_UC Bactoblood] project to enable freeze-drying of the system. | ||
Latest revision as of 15:49, 29 September 2009
Status: 500 Content-type: text/html
Software error:
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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Sequence codes for trehalose-6-phosphate synthase enzyme. This enzyme is the first of two required in the conversion of glucose to trehalose.
This enzyme catalyses the following reaction:
[http://en.wikipedia.org/wiki/Trehalose Trehalose] is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock otsa1, and is naturally produced in Escherichia Coli. We hope that by upregulating the trehalose production pathways in E.coli we can increase trehalose concentrations within our cell, thereby conferring some resistance to protein degredation in our system. This would allow easy transport and storage of the final product.
Usage and Biology
Transcription of OtsA gene is activated by osmotic stress in E. coli.otsa1
mRNA of OtsA is more stable at 16°C, therefore, it is a cold inducible mRNA.otsa2
OtsA mutation will block the synthesis of the trehalose-6-phosphase synthase, which is the enzyme that converts glucose-6-phosphate to trehalose-6-phosphate. otsa2
The gene was used alongside OtsB by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] team as part of the storage protection mechanism. It was also previously used by the UC Berkeley 2007 iGEM team as part of the [http://2007.igem.org/Berkeley_UC Bactoblood] project to enable freeze-drying of the system.
Sequence and Features
Status: 500
Content-type: text/html
Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 250. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
References
<biblio>
- otsa1 pmid=12105274
- otsa2 pmid=1310094
</biblio>