Difference between revisions of "Part:BBa K239000"

Revision as of 14:52, 29 September 2009

DegP promoter, activated by Phosporylated CpxR and SigmaE

Sequence contains 3 phosphorylated CpxR (Conjugative Plasmid eXpression) binding sites and transcription is initiated by sigma factor E. A native sequence has been used but it has been modified to remove Spe1 restriction site.

Function: Can detect various periplasmic stresses such as Copper (4mM CuCl2), Indole (4mM) and Ethanol (5%). The DegP protein is expressed by the cell to repair damage in the periplasm. We hope that it also can be used to detect presence of relevant unfolded proteins in the periplasmic space (e.g. target pharmaceuticals), to detect shear stress or other so far undefined relevant stress.

Note: DegP is dependent of Sigma E and can not be induced only by over expression of CpxR. In comparison, the Spy promoter, which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009)

Sequence and Features