Difference between revisions of "Part:BBa K4192131"
 
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===Biology===
 
===Biology===
 
<p>YF1 receives the light signal and transmits this signal to FixJ in the form of phosphorylation to regulate the opening and closing of the P<i>fixK2</i>(BBa_). promoter. </p>
 
<p>YF1 receives the light signal and transmits this signal to FixJ in the form of phosphorylation to regulate the opening and closing of the P<i>fixK2</i>(BBa_). promoter. </p>
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[[File:Yf1-p.png|600px|thumb|center|]]
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<p style="text-align: center;"><b>Fig.1 Regulatory mechanism of YF1 construction</b><sup>[https://pubmed.ncbi.nlm.nih.gov/19109976/]
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</sup>.</p>
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== References ==
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* Möglich, A., R.A. Ayers and K. Moffat, Design and Signaling Mechanism of Light-Regulated Histidine Kinases. Journal of Molecular Biology, 2009. 385(5): p. 1433-1444.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 17:11, 9 October 2022


yf1(N37C)-RBS-fixJ

Under light conditions, YF1 protein is excited and has kinase activity, which phosphorylates and activates FixL protein. Under dark conditions, YF1 protein does not phosphorylate FixL protein. This composite part is the key part that mainly converts light signals into intracellular signals. We mutated position 37 N of YF1 to C to improve its response to blue light and better regulate gene expression

Usage

This composition part serves as the signal conversion part of the photocontrol system, and its sensitivity to light determines the sensitivity of the photocontrol system to light. We designed the mutant yf1 (N37C) to expand the dynamic range of regulated gene expression under different signal intensities of the light control system, which would be more conducive to the abundant expression of downstream genes under dark conditions.

Biology

YF1 receives the light signal and transmits this signal to FixJ in the form of phosphorylation to regulate the opening and closing of the PfixK2(BBa_). promoter.

Yf1-p.png

Fig.1 Regulatory mechanism of YF1 construction[1] .

References

  • Möglich, A., R.A. Ayers and K. Moffat, Design and Signaling Mechanism of Light-Regulated Histidine Kinases. Journal of Molecular Biology, 2009. 385(5): p. 1433-1444.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1141
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1141
    Illegal NgoMIV site found at 544
    Illegal NgoMIV site found at 616
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 724
    Illegal NgoMIV site found at 1230
    Illegal NgoMIV site found at 1523
    Illegal NgoMIV site found at 1617
    Illegal AgeI site found at 258
    Illegal AgeI site found at 1398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1287
    Illegal BsaI.rc site found at 157