Difference between revisions of "Part:BBa K200001"

Latest revision as of 14:23, 29 September 2009

Dam methylase -> Dam

The methylase encoded by the dam gene ([http://en.wikipedia.org/wiki/Dam_(methylase) Dam methylase]) transfers a methyl group from S-adenosylmethionine to the N6 position of the adenine residues in the sequence GATC. When methylation occurs in the recognition site of a particular group of restriction endonuclease including MboI, this protects the DNA from cleavage.

Usage and Biology

DAM methylase (DNA adenine methylase) adds a methyl group to the adenine of the sequence 5'-GATC-3'in newly synthesized DNA. Dam methylases are important for several biological functions:

[http://en.wikipedia.org/wiki/DNA_replication DNA replication]
[http://en.wikipedia.org/wiki/Mismatch_repair DNA mismatch repair]
Regulation of protein expression.

In the E.ncapsulator project, Dam methylases are used to protect the DNA from being destroyed by restriction enzymes DpnII and TaqI.

Figure 1: Action of Dam methylase (source: #Dam1)

Figure: Action of Dam methylase (source: #Dam1)

The gene was used alongside with the restriction enzymes TaqI and the DpnII by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project as part of the genome deletion module. The Dam (methylase) is used to prevent genome restriction due to basal expression of the restriction enzymes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 306
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

<biblio>#Dam1 http://fangman-brewer.genetics.washington.edu/hemimethylation.html</biblio>

@@ Line 9: / Line 9: @@
 *[http://en.wikipedia.org/wiki/Mismatch_repair DNA mismatch repair]
 *Regulation of protein expression.
-In the E.ncapsulator project, Dam methylases are used to protect the DNA from being destroyed by restriction enzymes DpnII ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K200009 BBa_K200009]) and TaqI ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K200009 BBa_K200010]).
+In the E.ncapsulator project, Dam methylases are used to protect the DNA from being destroyed by restriction enzymes [[Part:BBa_K200009 |DpnII]] and [[Part:BBa_K200010 |TaqI]].
 [[Image:II09_dam.jpg|400px|centre|<i>Figure 1:</i> Action of Dam methylase (source: <cite>#Dam1</cite>)]]
 <center>Figure: Action of Dam methylase (source: <cite>#Dam1</cite>)</center>
-===Sequence and features===
+The gene was used alongside with the restriction enzymes [[Part:BBa_K200010 |TaqI]] and the [[Part:BBa_K200009 |DpnII]] by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project as part of the genome deletion module. The Dam (methylase) is used to prevent genome restriction due to basal expression of the restriction enzymes.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K200001 SequenceAndFeatures</partinfo>