Difference between revisions of "Part:BBa K200001"
(→‎Usage and Biology)
 
(3 intermediate revisions by 2 users not shown)
Line 9: Line 9:
 
*[http://en.wikipedia.org/wiki/Mismatch_repair DNA mismatch repair]
 
*[http://en.wikipedia.org/wiki/Mismatch_repair DNA mismatch repair]
 
*Regulation of protein expression.  
 
*Regulation of protein expression.  
−
In the E.ncapsulator project, Dam methylases are used to protect the DNA from being destroyed by restriction enzymes DpnII ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K200009 BBa_K200009]) and TaqI ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K200009 BBa_K200010]).
+
In the E.ncapsulator project, Dam methylases are used to protect the DNA from being destroyed by restriction enzymes [[Part:BBa_K200009 |DpnII]] and [[Part:BBa_K200010 |TaqI]].
  
 
[[Image:II09_dam.jpg|400px|centre|<i>Figure 1:</i> Action of Dam methylase (source: <cite>#Dam1</cite>)]]
 
[[Image:II09_dam.jpg|400px|centre|<i>Figure 1:</i> Action of Dam methylase (source: <cite>#Dam1</cite>)]]
 
<center>Figure: Action of Dam methylase (source: <cite>#Dam1</cite>)</center>
 
<center>Figure: Action of Dam methylase (source: <cite>#Dam1</cite>)</center>
  
−
===Sequence and features===
+
 
 +
The gene was used alongside with the restriction enzymes [[Part:BBa_K200010 |TaqI]] and the [[Part:BBa_K200009 |DpnII]] by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project as part of the genome deletion module. The Dam (methylase) is used to prevent genome restriction due to basal expression of the restriction enzymes.
 +
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K200001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K200001 SequenceAndFeatures</partinfo>

Latest revision as of 14:23, 29 September 2009

Status: 500 Content-type: text/html

Software error:

Not enough arguments for Range::range at /websites/parts.igem.org/cgi/lib/Range.pm line 250, near "range;"
Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_"
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_"
Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288.
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}"
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.


The methylase encoded by the dam gene ([http://en.wikipedia.org/wiki/Dam_(methylase) Dam methylase]) transfers a methyl group from S-adenosylmethionine to the N6 position of the adenine residues in the sequence GATC. When methylation occurs in the recognition site of a particular group of restriction endonuclease including MboI, this protects the DNA from cleavage.

Usage and Biology

DAM methylase (DNA adenine methylase) adds a methyl group to the adenine of the sequence 5'-GATC-3'in newly synthesized DNA. Dam methylases are important for several biological functions:

  • [http://en.wikipedia.org/wiki/DNA_replication DNA replication]
  • [http://en.wikipedia.org/wiki/Mismatch_repair DNA mismatch repair]
  • Regulation of protein expression.

In the E.ncapsulator project, Dam methylases are used to protect the DNA from being destroyed by restriction enzymes DpnII and TaqI.

Figure 1: Action of Dam methylase (source: #Dam1)
Figure: Action of Dam methylase (source: #Dam1)


The gene was used alongside with the restriction enzymes TaqI and the DpnII by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project as part of the genome deletion module. The Dam (methylase) is used to prevent genome restriction due to basal expression of the restriction enzymes.

Sequence and Features Status: 500 Content-type: text/html

Software error:

Not enough arguments for Range::range at /websites/parts.igem.org/cgi/lib/Range.pm line 250, near "range;"
Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_"
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_"
Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288.
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}"
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.


References

<biblio>#Dam1 http://fangman-brewer.genetics.washington.edu/hemimethylation.html</biblio>