Difference between revisions of "Part:BBa K4165000"

 
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part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein  
 
part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein  
  
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===Usage and Biology===
 
===Usage and Biology===
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Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with G of –8.8 and IC50 of 0.9 ± 0.1
  
 
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<partinfo>BBa_K4165000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165000 SequenceAndFeatures</partinfo>
  
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===Functional Parameters===
 
===Functional Parameters===
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Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
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cbeta_deviations = 0, clashscore = 69.77, molprobity = 4.26, ramachandran_favored = 40, ramachandran_outliers = 60, Qmean_4 = -6.45183, Qmean6 = -5.51397
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===Modeling===
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1b.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                  Figure 1.: 3D Model of H1A peptide Modelled by AlphaFold2.0 displayed in Pyomol
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===Docking===
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G=
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1b.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                  Figure 1.: 3D Model of H1A peptide Docked against HTRA1 displayed in Pyomol
 
<partinfo>BBa_K4165000 parameters</partinfo>
 
<partinfo>BBa_K4165000 parameters</partinfo>
 
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Revision as of 15:57, 9 October 2022


HTRA Binding Peptide 1

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with G of –8.8 and IC50 of 0.9 ± 0.1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0 cbeta_deviations = 0, clashscore = 69.77, molprobity = 4.26, ramachandran_favored = 40, ramachandran_outliers = 60, Qmean_4 = -6.45183, Qmean6 = -5.51397

Modeling

                 Figure 1.: 3D Model of H1A peptide Modelled by AlphaFold2.0 displayed in Pyomol

Docking

G= 

                 Figure 1.: 3D Model of H1A peptide Docked against HTRA1 displayed in Pyomol