Difference between revisions of "Part:BBa K4387001"
 
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__NOTOC__
 
 
<partinfo>BBa_K4387001 short</partinfo>
 
<partinfo>BBa_K4387001 short</partinfo>
  
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===Usage and Biology===
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==Usage and Biology==
  
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NorR is a transcriptional regulator of the nitric oxide sensitive promoter pNorV. This regulator was codon optimized for E.coli Nissle 1917 to avoid recombination. NorR acts as an activator of pNorV in the presence of nitric oxide and represses the expression of downstream genes otherwise[1].  
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NorR is a transcriptional regulator of the nitric oxide sensitive promoter pNorV. NorR acts as an activator of pNorV in the presence of nitric oxide and represses the expression of downstream genes otherwise[1]. This regulator was codon optimized for <i>E.coli Nissle 1917</i> to avoid recombination.  
  
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===Sequence and Features===
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This part is present in the following composite parts:
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<ul>
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<li><html><a href="https://parts.igem.org/Part:BBa_K4387003">Negative Control of Nitric Oxide Sensing Genetic Circuit</a></html></li>
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<li><html><a href="https://parts.igem.org/Part:BBa_K4387004">Nitric Oxide Sensing Genetic Circuit With the ETH promoter pNorV</a></html></li>
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<li><html><a href="https://parts.igem.org/Part:BBa_K4387005">Nitric Oxide Sensing Genetic Circuit With One Ribosomal Binding Site</a></html></li>
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<li><html><a href="https://parts.igem.org/Part:BBa_K4387006">Nitric Oxide Sensing Genetic Circuit With Two Ribosomal Binding Sites</a></html></li>
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<li><html><a href="https://parts.igem.org/Part:BBa_K4387007">Nitric Oxide Sensing Genetic Circuit With Three Ribosomal Binding Sites</a></html></li>
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</ul>
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==Sequence and Features==
 
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<span class='h3bb'/span>
 
<span class='h3bb'/span>
 
<partinfo>BBa_K4387001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4387001 SequenceAndFeatures</partinfo>
  
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==References==
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<ul>
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<li>[1] Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 <html><a href="https://pubs.acs.org/doi/abs/10.1021/acssynbio.1c00223">DOI: 10.1021/acssynbio.1c00223</a></html></li>
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</ul>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 15:53, 9 October 2022

Codon Optimized NorR

Usage and Biology

NorR is a transcriptional regulator of the nitric oxide sensitive promoter pNorV. NorR acts as an activator of pNorV in the presence of nitric oxide and represses the expression of downstream genes otherwise[1]. This regulator was codon optimized for E.coli Nissle 1917 to avoid recombination.

This part is present in the following composite parts:


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  • [1] Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223