Difference between revisions of "Part:BBa K200000"

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===Usage and Biology===
 
===Usage and Biology===
 
 Colanic acid is an exopolysaccharide secreted by some bacterial species to protect themselves from the environment that surrounds them.  
 
 Colanic acid is an exopolysaccharide secreted by some bacterial species to protect themselves from the environment that surrounds them.  
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<br><br>The gene was used alongside [[Part:BBa_K200002 |YgiV]] and [[Part:BBa_K200003 |Rfal]] as part of the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project to bind the colanic acid capsule to the cellular membrane. <br>
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<br><br>The gene was used alongside [[Part:BBa_K200002 |YgiV]] and [[Part:BBa_K200003 |Rfal]] by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project to upregulate colanic acid production as part of the auto-encapsulation mechanism. <br>
  
 
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Revision as of 09:49, 29 September 2009

Colanic acid global regulator -> RcsB

RcsB is a receiver protein in a two component phosphorelay system, which acts as a positive regulator of a number of genes including capsule genes responsible for colanic acid production. 2 This is via the activation of the ugd/cps operon which is required for capsule synthesis.
Colanic acid is a heteropolymer containing glucose, galactose, fucose, and glucuronic acid.

It is important to note that RcsB is a global regulator found in many [http://en.wikipedia.org/wiki/Enterobacteria Enterobacteria]. In Salmonella, up to 38 genes were found to be up-regulated by RcsB including ftsZ which affects the rate of cell division. 1 The majority of upregulated genes are associated with enhancing structural integrety and acid resistance. 22 genes were found to be downregualted by RcsB in the same microarray experiment.3

Usage and Biology

 Colanic acid is an exopolysaccharide secreted by some bacterial species to protect themselves from the environment that surrounds them.

The gene was used alongside YgiV and Rfal by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project to upregulate colanic acid production as part of the auto-encapsulation mechanism.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

<biblio>

  1. 1 pmid=1597415
  2. 2 pmid=2404948
  3. 3 pmid=19124574

</biblio>