Difference between revisions of "Part:BBa K4165124"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | A | + | A reversed sequence of a high affinity binding peptide is proposed for binding to the PDZ domain of HTRA1. |
We were motivated by our chosen Tau binding peptide since it was the inverted sequence of a high affinity peptide that shown better affinity, so we decided to try it with our HTRA1 BP. | We were motivated by our chosen Tau binding peptide since it was the inverted sequence of a high affinity peptide that shown better affinity, so we decided to try it with our HTRA1 BP. | ||
Revision as of 14:39, 9 October 2022
HTRA Binding Peptide 30
part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
Usage and Biology
A reversed sequence of a high affinity binding peptide is proposed for binding to the PDZ domain of HTRA1.
We were motivated by our chosen Tau binding peptide since it was the inverted sequence of a high affinity peptide that shown better affinity, so we decided to try it with our HTRA1 BP. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
cbeta_deviations = 0, clashscore = 16, molprobity = 3.73, ramachandran_favored = 20, ramachandran_outliers = 60, Qmean_4 = -5.832, Qmean6 = -3.92999
Modeling
Figure 1.: 3D Model of H2D peptide Modelled by AlphaFold2.0 displayed in Pyomol
References
Dammers, C., Yolcu, D., Kukuk, L., Willbold, D., Pickhardt, M., Mandelkow, E., ... & Funke, S. A. (2016). Selection and Characterization of Tau Binding ᴅ-Enantiomeric Peptides with Potential for Therapy of Alzheimer Disease. PLoS One, 11(12), e0167432.