Difference between revisions of "Part:BBa K4286099"
Line 5: Line 5:
 
2022 SZU-China has designed a timed suicide switch mediated by a classical synthetic gene oscillator. In the negative feedback loop, the oscillator is composed of three genes encoding repressor protein -- TetR from the Tn10 transposon, cI from bacteriophage lambda, and LacI from the lactose operon -- each repressor protein is attached to an LVA degradation tag located at the carboxy terminus. The concentration of each kind of repressor protein changed cyclically: lacI inhibited the expression of tetR, tetR inhibited the expression of cI, and cI inhibited lacI expression.
 
2022 SZU-China has designed a timed suicide switch mediated by a classical synthetic gene oscillator. In the negative feedback loop, the oscillator is composed of three genes encoding repressor protein -- TetR from the Tn10 transposon, cI from bacteriophage lambda, and LacI from the lactose operon -- each repressor protein is attached to an LVA degradation tag located at the carboxy terminus. The concentration of each kind of repressor protein changed cyclically: lacI inhibited the expression of tetR, tetR inhibited the expression of cI, and cI inhibited lacI expression.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
===Assembly===
 +
 +
===modeling===
 +
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 14:34, 9 October 2022


Classical oscillator composed of three genes encoding repressor protein

2022 SZU-China has designed a timed suicide switch mediated by a classical synthetic gene oscillator. In the negative feedback loop, the oscillator is composed of three genes encoding repressor protein -- TetR from the Tn10 transposon, cI from bacteriophage lambda, and LacI from the lactose operon -- each repressor protein is attached to an LVA degradation tag located at the carboxy terminus. The concentration of each kind of repressor protein changed cyclically: lacI inhibited the expression of tetR, tetR inhibited the expression of cI, and cI inhibited lacI expression.

Usage and Biology

Assembly

modeling

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]